Summarize and briefly explain the chemical interaction between EDC and NHS in Electrochemical Biosensing (CHEMICAL NAME: N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide)
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Q: briefly outline the evidence that methyl red and b- cyclodextrin formed a host- guest complex
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a. Summarize and briefly explain the chemical interaction between EDC and NHS in
b. Summarize and briefly explain the chemical interaction between Biotin & Avidin in Electrochemical Biosensing |
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- Briefly explain about Degradation of azo dyes , include aerobic, anaerobic etc.what the mechanism of action of EDTA in the study, advantages and results obtained.Describe the difference between an oil-in-water and a water-in-oil emulsion, and explain the use of the hydrophilic-lipophilic balance system to select an emulsifier.
- What are the nonprotein nitrogen components of the blood. Give their chemical structures and relative physiologic concentrations and describe their biosynthesis and excretion. Kindy insert your references. Thank you!2. A sample of material contains for its active components NaOH, NazCO3, NaHCO3, or compatible mixtures of these. Two samples, each weighing 1.000 g, are dissolved in H20. To one sample phenolphthalein is added and the solution is titrated cold with 1.038 N acid, of which 17.96 mL is required. The other sample is titrated cold, with methyl orange as indicator, and 21.17 mL of the same acid is required. What alkalis are present, and what is the percentage of each in the original sample?Give advantages of Ziegler–Natta catalysts ?
- a. At pH 4.4, there exists in solution equal amounts of the neutral and charged atropine molecules b. Atropine is a non-electrolyte drug c. Atropine releases its proton at pH 4.4 d. Atropine is most soluble at pH values higher than 4.4 e. If atropine is to be prepared in transdermal patches, the solution in the patch reservoir should have a pH less than 4.4 for maximum absorption.briefly outline the evidence that methyl red and b- cyclodextrin formed a host- guest complexWhich are true for polarography? I Polarography is amperometry conducted with a dropping Hg electrode. II The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III The half-wave potential for maximum current is characteristic of a given analyte in a given medium and can be used for qualitative analysis for the analyte. IV Fresh Hg drops give reproducible results. V Polarography is used to primarily to study oxidations Hg may reduce when studying reductions. A.I, III, and IV B. II, III, and V C. I and V D. III, IV, and V E. II, III, and IV
- One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained. ppm, P aBSORBANCE (A) 1.003.00 0.230 2.00 0.436 3.00 0.638 4.00 0.848 Urine sample 0.518 ffind the slope and intercept, then graph it.Describe and explain the effect(s) on your result for the following possible experimental errors. In each case, specify the component(s) whose percentage(s) would be too high or too low and explain your rationale why these would be changed. (a) After adding DCM to the counterfeit pharmaceutical at the beginning, you didn’t stir or shake the mixture before filtering. (b) Washing the sucrose recovered during the first vacuum filtration with water rather than DCM. (c) During the NaHCO3 extraction, you failed to mix the organic and aqueous layers thoroughly. - B (d) You mistakenly extracted the DCM solution with 5% HCl rather than 5% NaHCO3. (e) You neutralised the NaHCO3 solution to pH7 rather than pH2.In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging is performed.