The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular weight. a. If the tracking dye had moved a distance of 90.0 mm, calculate linear regression parameters such as linearity coefficient (r), the slope (m), and the y-intercept (b). Protein MW (Da Distance moved (mm) Transferrin 78 000 6.0 12.5 Bovine serum albumin 66 000 Ovalbumin 45 000 32.0 Trypsinogen Myoglobin Lysozyme Cytochrome c 24 000 54.0 17 800 69.0 14 300 79.0 12 400 86.5 Proliferating cell nuclear antigen (PCNA) acts as a homotrimer of three identical subunits and helps increase the processivity of leading strand synthesis during DNA replication. It has a molecular weight of 36.0 kDa. Under the reducing conditions of the sample buffer, the disulfide bridges linking the subunits are reduced and thus the monomer chains are seen on the gel. b. Calculate the Rf of the single band of PCNA when subjected to SDS-PAGE. c. Predict the distance (in mm) that will be traveled by the band. Report your answer in one (1) decimal place.
The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular weight. a. If the tracking dye had moved a distance of 90.0 mm, calculate linear regression parameters such as linearity coefficient (r), the slope (m), and the y-intercept (b). Protein MW (Da Distance moved (mm) Transferrin 78 000 6.0 12.5 Bovine serum albumin 66 000 Ovalbumin 45 000 32.0 Trypsinogen Myoglobin Lysozyme Cytochrome c 24 000 54.0 17 800 69.0 14 300 79.0 12 400 86.5 Proliferating cell nuclear antigen (PCNA) acts as a homotrimer of three identical subunits and helps increase the processivity of leading strand synthesis during DNA replication. It has a molecular weight of 36.0 kDa. Under the reducing conditions of the sample buffer, the disulfide bridges linking the subunits are reduced and thus the monomer chains are seen on the gel. b. Calculate the Rf of the single band of PCNA when subjected to SDS-PAGE. c. Predict the distance (in mm) that will be traveled by the band. Report your answer in one (1) decimal place.
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter13: Artifacts And Noise In Medical Instrumentation
Section: Chapter Questions
Problem 7P
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Transcribed Image Text:The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker
proteins of known relative molecular weight.
a. If the tracking dye had moved a distance of 90.0 mm, calculate linear regression parameters such as
linearity coefficient (r), the slope (m), and the y-intercept (b).
Protein
MW (Da
Distance moved (mm)
Transferrin
78 000
6.0
Bovine serum albumin
66 000
12.5
Ovalbumin
45 000
32.0
54.0
Trypsinogen
Myoglobin
Lysozyme
Cytochrome c
24 000
17 800
69.0
14 300
79.0
12 400
86.5
Proliferating cell nuclear antigen (PCNA) acts as a homotrimer of three identical subunits and helps
increase the processivity of leading strand synthesis during DNA replication. It has a molecular weight
of 36.0 kDa. Under the reducing conditions of the sample buffer, the disulfide bridges linking the
subunits are reduced and thus the monomer chains are seen on the gel.
b. Calculate the Rf of the single band of PCNA when subjected to SDS-PAGE.
c. Predict the distance (in mm) that will be traveled by the band. Report your answer in one (1) decimal
place.
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