The function of the ten subunits of deoxyribonucleic acid (DNA) polymerase III and differences between holoenzyme and the core enzyme. Introduction: DNA polymerase III is the primary enzyme complex involved in DNA replication of both leading as well as lagging strand in prokaryotes like Escherichia coli ( E. coli ). It is a holoenzyme that contains ten subunits. All these subunits have their own specific functions.

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 10, Problem 13PDQ

Summary Introduction

To review:

The function of the ten subunits of deoxyribonucleic acid (DNA) polymerase III and differences between holoenzyme and the core enzyme.

Introduction:

DNA polymerase III is the primary enzyme complex involved in DNA replication of both leading as well as lagging strand in prokaryotes like Escherichia coli (E. coli). It is a holoenzyme that contains ten subunits. All these subunits have their own specific functions.

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List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.

Explain the molecular mechanism of DNA polymerization by DNA polymerase and explain why DNA polymerase 3 and not dna polymerase 1 is responsible for replicating the bacterial genome.

Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a detailed explanation.

Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 10, Problem 13PDQ

Summary Introduction

To review:

The function of the ten subunits of deoxyribonucleic acid (DNA) polymerase III and differences between holoenzyme and the core enzyme.

Introduction:

DNA polymerase III is the primary enzyme complex involved in DNA replication of both leading as well as lagging strand in prokaryotes like Escherichia coli (E. coli). It is a holoenzyme that contains ten subunits. All these subunits have their own specific functions.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 10, Problem 13PDQ

Summary Introduction

To review:

The function of the ten subunits of deoxyribonucleic acid (DNA) polymerase III and differences between holoenzyme and the core enzyme.

Introduction:

DNA polymerase III is the primary enzyme complex involved in DNA replication of both leading as well as lagging strand in prokaryotes like Escherichia coli (E. coli). It is a holoenzyme that contains ten subunits. All these subunits have their own specific functions.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 10, Problem 13PDQ

Summary Introduction