Pearson eText for Biochemistry: Concepts and Connections -- Instant Access (Pearson+)
2nd Edition
ISBN: 9780137533114
Author: Dean Appling, Spencer Anthony-Cahill
Publisher: PEARSON+
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Chapter 10, Problem 20P
The transport of aspirin (pKa = 3.5, structure shown here) form the digestive tract to the circulation occurs by nonmediated absorption into cells lining the stomach (where pH=0.8) and the small intestine (where pH=6.0). Do you expect absorption to be faster in the stomach or in the small intestine?
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The acidity of the stomach is maintained by the H*/K* ATPase in parietal cells of the gastric mucosa.
These cells have an internal pH = 7.4. The H*/K* ATPase transports H* across the cellular membrane into the
stomach where pH = 0.8.
(a) Given a membrane potential of 65 mV (inside negative), calculate AG in kJ/mol for the transport of H* into the
stomach at 37 °C. Show your work.
(b) The hydrolysis of ATP is directly coupled to H* transport and the stoichiometry of the transport reaction is such
that 1 mol of ATP is hydrolyzed for every 1 mol of H* transported into the stomach. ATP is hydrolyzed by the
ATPase on the inside of the cell. Under the conditions given in part (a) and assuming the steady-state
concentrations of ATP, ADP and P₁ given below, is the transport of H* in to the stomach thermodynamically
favorable at 37 °C? Show the calculation that supports your answer.
Steady-state intracellular concentrations:
ATP = 4.2 mM
ADP = 360 μM
Pi = 5.4 mM
For ATP hydrolysis at 37 °C: ATP…
The phosphate buffer (H2PO4 HPO42+ H") in esophageal cells has a pKa of 7.2. If acid floods into
cells, and the pH drops from 7.2 to 3.2, which concentration in the cells will increase, H2PO4 or HPO42?
Is pH 3.2 in the buffer range of this phosphate buffer (pKa 7.2)?
Esophagus
Stomach
HPO42, no
HPO4: yes
H,PO4: yes
H,PO41; no
Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The
total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4
* 10° positively charged amine groups that can adsorba negatively charged protein that passes through the
column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion
exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups
on DEAE cellulose is 7.1, and the pl of the protein is 5.6.
2.
A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE
are bound to the protein through an ionic bond)? You may assume that one protein attaches to one +
charge on the beads (although it's possible that proteins attach to more than one + charge).
B. After reaching 80% capacity, explain what you would do to release the protein attached to the…
Chapter 10 Solutions
Pearson eText for Biochemistry: Concepts and Connections -- Instant Access (Pearson+)
Ch. 10 - Prob. 1PCh. 10 - Given these molecular components--glycerol, fatty...Ch. 10 - The classic demonstration that cell plasma...Ch. 10 - The lipid portion of a typical bilayers is about...Ch. 10 - In the following situations, what is the free...Ch. 10 - Propose an experiment that would distinguish...Ch. 10 - Prob. 7PCh. 10 - Peptide hormones (such as insulin) must bind to...Ch. 10 - Prob. 9PCh. 10 - Prob. 10P
Ch. 10 - Prob. 11PCh. 10 - Prob. 12PCh. 10 - Prob. 13PCh. 10 - Prob. 14PCh. 10 - The concentration of glucose in your circulatory...Ch. 10 - ATP is synthesized from ADP, Pi , and a proton on...Ch. 10 - The Na+/ glucose symport transports glucose from...Ch. 10 - Prob. 18PCh. 10 - Prob. 19PCh. 10 - The transport of aspirin (pKa = 3.5, structure...Ch. 10 - Prob. 21P
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- In a TPN bag contains: Kcal of 1000 ml of D50 W, 1000 ml of 7% amino acids, and 650 ml of 20% lipids. A. How many total kcal is provided by this bag of TPN?arrow_forwardThe parietal cells of the stomach lining contain membrane “pumps” that transport hydrogen ions from the cytosol (pH 7.0) into the stomach,contributing to the acidity of gastric juice (pH 1.0). Calculate the free energy required to transport 1 mol of hydrogen ions through these pumps. Assume a temperature of 37 °C.arrow_forwardA mixture of Alanine (pl 6.02), Glutamic Acid (pl 3.22), Glycine (pl 5.79), Lysine (pl 9.74) and Threonine (pl 6.53) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2 ? v First 1. Alanine v Second 2. Glutamic Acid v Third 3. Glycine v Fourth 4. Lysine v Fifth 5. Threonine 6. No separationarrow_forward
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