Biology: Science for Life with Physiology (6th Edition) (Belk, Border & Maier, The Biology: Science for Life Series, 5th Edition)
6th Edition
ISBN: 9780134555430
Author: Colleen Belk, Virginia Borden Maier
Publisher: PEARSON
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Chapter 10, Problem 3AAATB
Summary Introduction
To write:
The site at which the restriction enzymes cuts the DNA.
Introduction:
Genome is defined as the complete genetic material that is present in an organism. It consists of the coding as well as the non-coding parts of DNA. The study of the genome is termed as genomics. Restriction enzymes are widely used enzymes in the field of genomics. This enzyme was discovered by three scientists named “Werner Arber, Hamilton Smith, and Daniel Nathans”.
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Students have asked these similar questions
Which of the following sequences, when combined with itscomplement, would be clipped by a restriction endonuclease?a. ATCGATCGTAGCTA
b. AAGCTTCGAA
c. GAATTC
d. ACCATTGGA
Choose the combination of answers that most accurately completes the statement.Which of the following sequences, when combined with its complement, would be clipped by a restriction endonuclease? a. ATCGATCGTAGCTA c. GAATTC b. AAGCTTCGAA d. ACCATTGGA
If a restriction enzyme cuts at this DNA sequence: TACGGAT, in general what is this sequence called?
Chapter 10 Solutions
Biology: Science for Life with Physiology (6th Edition) (Belk, Border & Maier, The Biology: Science for Life Series, 5th Edition)
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- Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme? AAGG TTCC GGCC CCGG ACCA TGGT AAAA TTTTarrow_forwardWhich of the following sequences, in the double-stranded form, can probably be recognized by a restriction enzyme? Choose an answer from below? TTCCTT CGACGA GTCGAC GGTTGG GAGGACarrow_forwardUse the sequence provided and make use of figure 1 to determine what restriction enzyme uses the spesific recognition site and figure 2 and 3 to detremine how many times does the sequence occur in the λ DNA sequence? GGATCC: __________________________________________________________ GAATTC: ___________________________________________________________ AAGCTT: ___________________________________________________________arrow_forward
- Which of the following is a sequence at which a restriction enzyme would cut? A) 5'AAGCAA3' B) 5'AAGCTT3' C) 5'ACAACA3' D) 5'AGAGAG3' E) 5'AGCCGT3' F) 5'AATTAAC3'arrow_forwardName the mapping technique used to determine the position of restriction sites in a DNA molecule.arrow_forwardUsing the restriction site for SalI, suggest another restriction enzyme that would cut at the same site.arrow_forward
- You want to insert a sequence in the lacI gene to alter its function,what restriction enzyme(s) will you use?Indicate 1 or 2restriction enzyme(s) that you will use on the gene to show the cutarrow_forwardAlthough SINES are nonautonomous, they do occasionally move from one genomic site to another. How is this usually achieved? a. Using another TE's insertion sequence b. Using another TE's restriction enzyme c. Using another TE's transposase d. Using another TE's reverse transcriptasearrow_forwardIf the following is a restriction enzyme: Sma I, A. What is the first letter represent? B. What do the next two letters represent? C. What does the roman numeral represent?arrow_forward
- Which of the following sequences in double-stranded DNA ismost likely to be recognized as a cutting site for a restrictionenzyme?(A) AAGGTTCC(B) GGCCCCGG(C) ACCATGGT(D) AAAATTTTarrow_forwardWhy would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA?arrow_forwardWhich of the two restriction sites in pQE vector will be appropriate to allow full length insertion of your gene? These restriction sites will serve as linkers on both ends of your genearrow_forward
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