BIOLOGY DUAL ENROLLMENT VERSION
BIOLOGY DUAL ENROLLMENT VERSION
12th Edition
ISBN: 9780136681311
Author: Urry
Publisher: PEARSON
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Chapter 10.4, Problem 5CC
Summary Introduction

To find: The role of glyceraldehyde 3-phosphate (G3P) in glycolysis and Calvin cycle.

Introduction:

Glycolysis is a stepwise process by which one molecule of glucose breaks into two molecules of pyruvic acid by partially oxidizing glucose. This process occurs in the cytoplasm of the cell.

Calvin cycle is a biochemical pathway for the reduction of CO2 or photosynthetic carbon. The acceptor molecule is a 5-carbon ketoses sugar i.e. Ribulose 5-bisphosphate (5 RuBP).

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Please explain each part thoroughly with explanations and answers given. This is Bipchemistry
make a schematic diagram of BChE/AChE inhibitory determination Butyrylthiocholine iodide (BChI), as well as acetylthiocholine iodide(AChI) molecules were utilized as substrate compounds of the reaction. Inthis regard, 5,5′‐dithio‐bis(2‐nitro‐benzoic acid) compound (DTNB) wasused to estimate BChE/AChE activity. In summary, 100 μL Tris buffersolution (HCl, 1.0M, pH 8.0, Tris) and variety of concentrations of thesample solution (50‐200 mL) were added in deionized water and also20 μL of BChE/AChE enzymes solutions added. The mixture was thenincubated for 10 minutes at 20°C. Finally, 50 μL of DTNB (0.5 mM and25 mL) BChI/AChI were added to the incubator mixture. The reactionwas also started by adding 50 μL BChI/AChI. The activity of theseenzymes was evaluated using spectrophotometric spectra with awavelength of 412 nm.
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