Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 11, Problem 12TF
Summary Introduction
Introduction:
High temperature ranges are considered as microbicidal that kill most of the microorganisms. Boiling water, pasteurization, non pressurized heat and autoclave are the methods of moist heat control.
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The standard temperature-pressure combination for an autoclave isa. 100°C and 4 psi
b. 121°C and 15 psi
c. 131°C and 9 psi
d. 115°C and 3 psi
With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?
Choose the combination of answers that most accurately completes the statement. The temperature-pressure combination for an autoclave is a. 1008C and 4 psi c. 1318C and 9 psi b. 1218C and 15 psi d. 1158C and 3 psi
Chapter 11 Solutions
Microbiology: A Systems Approach
Ch. 11.1 - Distinguish among the terms sterilization,...Ch. 11.1 - Prob. 2AYPCh. 11.1 - Prob. 3AYPCh. 11.1 - Name four categories of cellular targets for...Ch. 11.2 - Prob. 5AYPCh. 11.2 - Prob. 6AYPCh. 11.2 - Define thermal death time and thermal death point,...Ch. 11.2 - Prob. 8AYPCh. 11.2 - Prob. 9AYPCh. 11.2 - Identify advantages and disadvantages of cold...
Ch. 11.2 - Differentiate between the two types of radiation...Ch. 11.2 - Prob. 12AYPCh. 11.2 - Prob. 13AYPCh. 11.3 - Name the desirable characteristics of chemical...Ch. 11.3 - Discuss several different halogen agents and their...Ch. 11.3 - List advantages and disadvantages to the use of...Ch. 11.3 - Explain the mode of action of alcohols and their...Ch. 11.3 - Prob. 18AYPCh. 11.3 - Define the term surfactant, and explain this...Ch. 11.3 - Identify examples of some heavy metal control...Ch. 11.3 - Discuss the advantages and disadvantages of...Ch. 11.3 - Prob. 22AYPCh. 11 - Microbial control methods that kill/destroy...Ch. 11 - Sanitization is a process by which a. the...Ch. 11 - Prob. 3MCQCh. 11 - Prob. 4MCQCh. 11 - Prob. 5MCQCh. 11 - The primary mode of action of nonionizing...Ch. 11 - The most practical method of disinfecting...Ch. 11 - A chemical with sporicidal properties is a....Ch. 11 - Prob. 9MCQCh. 11 - Detergents are a. high-level germicides. b....Ch. 11 - Prob. 11TFCh. 11 - Prob. 12TFCh. 11 - Prob. 13TFCh. 11 - Prob. 14TFCh. 11 - Prob. 15TFCh. 11 - a.Precisely what is microbial death? b.Why does a...Ch. 11 - Prob. 2CTQCh. 11 - Prob. 3CTQCh. 11 - Tissue transplantation is a procedure that saves...Ch. 11 - Conduct additional research on the use of...Ch. 11 - From chapter 2, figure 2.20. Study this...Ch. 11 - Prob. 2VCCh. 11 - Using the words that follow, please create a...
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- The autoclave uses a combination of ___ to accomplish its function: a) temperature and pressure b) chemical and thermal parameters c) Gas and pressure d) antimicrobial and inorganic parametersarrow_forwardHow would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.arrow_forwardIn your opinion and based on your knowledge, what is the most important component that will be tested using the reagent test strips?arrow_forward
- What is the point of the pressure in the autoclave sterilization?arrow_forwardWhat will pose an ethical issue in these procedures are performed - sterilization?arrow_forwardIn autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?arrow_forward
- Serial dilution is recommended prior to pour and spread plate. Why is this so?arrow_forwardThe thermal death time in an autoclave at 121oC for a microbial culture was thirteen minutes. The thermal death time for another sample of the same culture in a hot-air oven at 121oC was twenty-one minutes. Use your knowledge of what thermal death time measures to identify which method of heating was more effective against this microbial culture. The hot-air oven treatment was more effective. The autoclave treatment was more effective. Both thermal death times were equally effective. Not enough information is provided to determine effectiveness.arrow_forwardWhat is pyrogen ?How to get rid of the pyrogen from the formulation? Please answer at your own easy words. Answer should be to the point.arrow_forward
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