ESSENTIALS OF GENETICS ALC & MOD MSTG/ET VP
1st Edition
ISBN: 9780134452890
Author: KLUG
Publisher: Pearson Education
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Chapter 11, Problem 16PDQ
Summary Introduction
To review:
The viral DNA having the length of 50 μm and the diameter of 20 Å, and whether the genome will fit into the viral head of 0.08 Å diameter or not.
Introduction:
The virus is described as the entities that perform all the biological activities inside the host cell. The genome of the virus is tightly packed inside the small head of the virus. This is achieved by the packaging of the genome in the condensed form. Different proteins are involved in the packaging of the genome in the head of the virus.
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A biological motor used to package double-stranded (ds) DNA into viral capsids is able toexert 50 pN of force.
A. If the active site has a surface area of 10^-17 m2 , to what pressure can the biological motor package dsDNA within the viral capsid before stalling?
B.If the viral capsid has a radius of 30 nm, how much potential energy is stored due to the pressure-confinement of the dsDNA within the capsid?
C. Compare this to thermal energy at room temperature, kBT, where kB=1.38*10^-23 J/K is the Boltzmann constant.
The base composition of an RNA virus was analyzed and found to be 14.1% A, 14.0% U, 36.2% G, and 35.7% C. Would you conclude that the viral genetic material is single-stranded RNA or doublestranded RNA?
RNA was extracted from certain virus and found to contain 35% cytosine. With this information, is it possible to predict what percentage of the bases in virus are adenine? If so, what percentage? If not, why not?
Chapter 11 Solutions
ESSENTIALS OF GENETICS ALC & MOD MSTG/ET VP
Ch. 11 - CASE STUDY | Art inspires learning A genetics...Ch. 11 - Prob. 2CSCh. 11 - Prob. 3CSCh. 11 -
HOW DO WE KNOW?
1. In this chapter, we focused on...Ch. 11 - Review the Chapter Concepts list on p. 199. These...Ch. 11 - Prob. 3PDQCh. 11 - Describe how giant polytene chromosomes are...Ch. 11 - What genetic process is occurring in a puff of a...Ch. 11 - Prob. 6PDQCh. 11 - Why might we predict that the organization of...
Ch. 11 -
8. Describe the sequence of research findings...Ch. 11 - Prob. 9PDQCh. 11 - Prob. 10PDQCh. 11 - Provide a comprehensive definition of...Ch. 11 - Prob. 12PDQCh. 11 - Define satellite DNA. Describe where it is found...Ch. 11 - Prob. 14PDQCh. 11 -
15. Mammals contain a diploid genome consisting...Ch. 11 - Prob. 16PDQCh. 11 - Prob. 17PDQCh. 11 - Prob. 18PDQCh. 11 - Prob. 19PDQCh. 11 - The human genome contains approximately 106 copies...Ch. 11 - Prob. 21PDQ
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- What percent of guanine exist in virus genome if it contained 35% if adenine in double stranded RNA genome after isolating it from a virus?arrow_forwardGiven the following DNA sequence: 5’-ATGCGGCCAAGGTCAGAGTGACA-3’ a) If this DNA strand represents the “Sense Strand” of DNA, what would be the RNA sequence? b) If this DNA strand represents the “Antisense Strand” of DNA, what would be the RNA Sequence? c) What would be the other strand of DNA?arrow_forwardThe chromosome of the bacterium (prokaryote) Escherichia coli has a size of approximately 4,000 kilobases (kb) [one kilobase = 1,000 bases]. What length of DNA (B form) would be contained in it? How does this length compare to the average size and/or volume of this bacterium?arrow_forward
- The principal genomic component isolated from equine influenza virus is 22% C, 23% A, 22% G and 33% U. Is this sufficient data to determine whether the genomic material is DNA/RNA and is double- or single-stranded? Why or why not? Explain, using a diagramarrow_forwardYou digest a 5 kb plasmid with two enzymes that yield fragments of 4 kb and 1 kb. After running the digest on a gel, what would you predict to see in terms of the intensities of the bands?arrow_forwardFor linear 34,000 kb DNA calculate a) contour length; b) the length of DNA as packaged in nucleosomes and c) the length of the DNA in a 30-nm fiber.arrow_forward
- You are studying RNA viruses and have discovered one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don't know if it is positive or negative stranded. Your lab-mate says, "Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome." You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate's suggestion?arrow_forwardYou have 2 solutions of DNA. Solution 1 contains single stranded viral DNA while Solution 2 has double stranded form of the same viral DNA. Both solutions contain 1 mg/ml of DNA. You then expose each of these solutions to UV light at 260 nm. Which of the following results would you expect to see after UV light exposure? a.Neither solution will absorb any UV light but the DNA in both solutions will be broken by the action of the UV light. b.Solution 1 will absorb less light than Solution 2 c.Solution 1 will absorb more light than solution 2. d.Nothing will occur. DNA can only absorb UV light if it is interchelated with ethidium bromide. e.Both solutions will absorb the same amount of light since their concentrations are the same.arrow_forwardThe base composition of an RNA virus was analyzed and found tobe 14.1% A, 14.0% U, 36.2% G, and 35.7% C. Would you concludethat the viral genetic material is single-stranded RNA ordouble-stranded RNA?arrow_forward
- Total nucleic acids are extracted from a growing culture of yeast cells. They are then mixed with specialized beads to which the single-stranded DNA molecule with sequence 5’-TTTTTTTTTTTTTTTTTTTTTTTTT-3’ has been covalently attached to the surface (see image to the right, where each black line represents a polynucleotide sequence). After a short incubation time, the beads are removed from the mixture. When you analyze the cellular nucleic acids stuck to the beads, which type of nucleic acid (i.e. DNA, rRNA, etc.) do you expect to be the most abundant? Why?arrow_forwardLet’s assume the linker region of DNA averages 54 bp in length. How many molecules of H2A would you expect to find in a DNA sample that is 46,000 bp in length?arrow_forwardYou are studying RNA viruses and have discovered a new one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don’t know if it is plus or minus stranded. Your lab-mate says, “Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome.” You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate’s suggestion?arrow_forward
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