EP CONNECT ONLINE ACCESS FOR BIOLOGY:
5th Edition
ISBN: 9781260542226
Author: Hoefnagels
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 11, Problem 1PIT
Review the Survey the Landscape figure in the chapter introduction. Given DNA’s role in the cell, why do the basic tools of DNA technology summarized in figure l l.20 have applications in such diverse fields of study?
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Researchers can isolate a sample of cells, such as skin fibroblasts, and grow them in the laboratory. This procedure is called a cell culture. A cell culture can be exposed to a sample of DNA. If the cells are treated with agents that make their membranes permeable to DNA, the cells may take up the DNA and incorporate it into their chromosomes. This process is called transformation or transfection. Scientists have transformed human skin fibroblasts with methylated DNA and then allowed the fibroblasts to divide for several cellular generations. The DNA in the daughter cells was then isolated, and the segment that corresponded to the transformed DNA was examined. This DNA segment in the daughter cells was also found to be methylated. However, if the original skin fibroblasts were transformed with unmethylated DNA, the DNA found in the daughter cells was also unmethylated. With regard to the transformed DNA, do fibroblasts perform de novo methylation, maintenance methylation, or both?…
Chapter 11 Solutions
EP CONNECT ONLINE ACCESS FOR BIOLOGY:
Ch. 11.1 - Prob. 1MCCh. 11.1 - Prob. 2MCCh. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - Prob. 2MCCh. 11.2 - Prob. 3MCCh. 11.2 - What is the function of the 98.5% of the human...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - Prob. 6MCCh. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Prob. 1MC
Ch. 11.3 - Prob. 2MCCh. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Prob. 4MCCh. 11.4 - Prob. 1MCCh. 11.4 - Prob. 2MCCh. 11.4 - Prob. 3MCCh. 11.4 - Prob. 4MCCh. 11.4 - What are some examples of ethical questions raised...Ch. 11.5 - Prob. 1MCCh. 11.5 - Prob. 2MCCh. 11 - If a restriction enzyme cuts between the G and the...Ch. 11 - Which of the following is not a reason that...Ch. 11 - Prob. 3MCQCh. 11 - Prob. 4MCQCh. 11 - Prob. 5MCQCh. 11 - Prob. 6MCQCh. 11 - Prob. 7MCQCh. 11 - What techniques might researchers use to create...Ch. 11 - Prob. 2WIOCh. 11 - Prob. 3WIOCh. 11 - Prob. 4WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - In a 2013 investigation, researchers discovered...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Prob. 8WIOCh. 11 - Prob. 9WIOCh. 11 - Prob. 10WIOCh. 11 - If a cells genome is analogous to a cookbook and a...Ch. 11 - Prob. 12WIOCh. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR relate to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...
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- According to our class discussions, which of the following is the most correct definition of a mutation? a. A permanent, heritable change in DNA base sequence b. Changes in the form of DNA packaging used in the nucleus C. Damage to DNA caused by random chemical reactions inside cells d. Damage to DNA bases caused by environmental factors, such as UV light or cigarette smokearrow_forwardThere are three basic steps of DNA extraction: cell lysis, separation, and extraction. In the procedure for extracting DNA from human cheek cells, which parts belong to or explain the aforementioned three basic steps? You may refer to the following procedure: http://www.planet-science.com/categories/experiments/biology/2012/03/extract-your-own-dna.aspxarrow_forwardYou are provided with a sample of aardvark DNA. As part of your investigation of this DNA, you transcribe mRNA from the DNA and purify it. You then separate the two strands of the DNA and analyze the base composition of each strand and of the mRNA transcripts. You obtain the following results: A G C T UDNA strand #1 19.1 26.0 31.0 23.9 0DNA strand #1 24.2 30.8 25.7 19.3 0mRNA 19.0 25.9 30.8 0 24.3Which strand of the DNA is the “sense” strand that serves as the template for mRNA synthesis?arrow_forward
- Once separated from other cellular components, extracted DNA can be used for a variety of purposes. Name 1 use of human DNA that is common today.arrow_forwardAnswer the question in the attached picture:arrow_forwardTo promote genetic diversity in bacteria, it has been found that some species have a genetic mechanism that allows them to increase their mutation rate during DNA replication. A scientist hypothesizes that the functions of the following two enzymes would be impacted by this mechanism. In each case, state if you agree and provide a reason for your answer. i) DNA primase ii) DNA polymerase IIIarrow_forward
- For each statement below indicate whether it is true or false: A. Strand discrimination during the process of DNA repair is based on DNA methylation B. The genetic code has 64 amino acid-specifying codons C. Mutations in germline cells are more consequential to subsequent generations than mutations in somatic cells.arrow_forwardAnswer Part d,e,farrow_forwardRefer to the DNA models below. a. In what ways are they similar?b. In what ways are they different?arrow_forward
- Briefly explain about computer-automated high-throughput DNA sequencing ?arrow_forwardPlace the following steps in order to outline how enzymes are involved with proofreading newly formed DNA molecules. 1. DNA polymerase does not detect damaged DNA. 2. Ligase connects the free end of the new DNA with the old DNA. 3. DNA polymerase replaces the damaged DNA with the correct nucleotide. 4. Exonuclease cuts the damaged DNA strand in order to remove the damaged section.arrow_forwardRead the images given before answering the question below. Identify the two common diseases that result from mutations in gene A?arrow_forward
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