EP CONNECT ONLINE ACCESS FOR BIOLOGY:
5th Edition
ISBN: 9781260542226
Author: Hoefnagels
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Question
Chapter 11, Problem 4WIO
Summary Introduction
To differentiate:
Between the use of the DNA polymerase enzyme in DNA sequencing and PCR.
Concept introduction:
DNA polymerase enzymes are important enzymes that are involved in the replication of DNA. It helps in the elongation of the DNA strands, repair mechanisms as well as in the proofreading processes.
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Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What are some of the limitations of PCR?
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Chapter 11 Solutions
EP CONNECT ONLINE ACCESS FOR BIOLOGY:
Ch. 11.1 - Prob. 1MCCh. 11.1 - Prob. 2MCCh. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - Prob. 2MCCh. 11.2 - Prob. 3MCCh. 11.2 - What is the function of the 98.5% of the human...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - Prob. 6MCCh. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Prob. 1MC
Ch. 11.3 - Prob. 2MCCh. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Prob. 4MCCh. 11.4 - Prob. 1MCCh. 11.4 - Prob. 2MCCh. 11.4 - Prob. 3MCCh. 11.4 - Prob. 4MCCh. 11.4 - What are some examples of ethical questions raised...Ch. 11.5 - Prob. 1MCCh. 11.5 - Prob. 2MCCh. 11 - If a restriction enzyme cuts between the G and the...Ch. 11 - Which of the following is not a reason that...Ch. 11 - Prob. 3MCQCh. 11 - Prob. 4MCQCh. 11 - Prob. 5MCQCh. 11 - Prob. 6MCQCh. 11 - Prob. 7MCQCh. 11 - What techniques might researchers use to create...Ch. 11 - Prob. 2WIOCh. 11 - Prob. 3WIOCh. 11 - Prob. 4WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - In a 2013 investigation, researchers discovered...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Prob. 8WIOCh. 11 - Prob. 9WIOCh. 11 - Prob. 10WIOCh. 11 - If a cells genome is analogous to a cookbook and a...Ch. 11 - Prob. 12WIOCh. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR relate to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...
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- Explain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forwardDescribe what occurs in each of the three steps during the PCR reaction.arrow_forwardFrom a single DNA molecule, calculate how many copies would be produced in 12 cycles of pcrarrow_forward
- Compare and contrast traditional PCR with quantitative PCR.arrow_forwardDefine PCR. Write down different types of PCR in detail with their applications. Give the answer broadly.arrow_forwardExplain the role of different polymerases in PCR with their advantages and limitations. Please explain at your own words.arrow_forward
- Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.arrow_forwardBriefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.arrow_forwardOutline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a genome.arrow_forward
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