Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 11, Problem 26P
Experimental evidence demonstrates that the nucleosomes present in a cell after the completion of S phase are composed of some “old” histone dimers and some newly synthesized histone dimers. Describe the general design for an experiment that uses a protein label such as
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A diploid human cell contains approximately 6.4 billion base pairs of DNA.
Assuming that the linker DNA encompasses 35 bp, how many nucleosomes are present in such a cell? Use two significant figures. How many histone proteins are complexed with this DNA? use two significant figures.
Describe the packaging of chromosomal DNA by histones with diagrammatic representations. Name the various histone modifications and describe any two among them.
A diploid human cell contains approximately 6.4 billion base pairs of DNA. a. How many nucleosomes are present in such a cell? (Assume that the linker DNA encompasses 40 bp.) b. How many histone proteins are complexed with this DNA?
Chapter 11 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 11 - Prob. 1PCh. 11 - Prob. 2PCh. 11 - Bacterial DNA is compacted by two principal...Ch. 11 - 10.2 The human genome contains contains base...Ch. 11 - 10.1 Give descriptions for the following...Ch. 11 - 10.4 Describe the importance of light and dark G...Ch. 11 - In eukaryotic DNA, Where are you most likely to...Ch. 11 - Prob. 8PCh. 11 - Human late prophase karyotypes have about 2000...Ch. 11 - 10. What are the two or three most essential...
Ch. 11 - Prob. 11PCh. 11 - Prob. 12PCh. 11 - A researcher interested in studying a human gene...Ch. 11 - Prob. 14PCh. 11 - 10.11 In what way does position effect variegation...Ch. 11 - 16. What are chromosome territories, and what...Ch. 11 - Prob. 17PCh. 11 - Prob. 18PCh. 11 - 10.18 A survey of organisms living deep in the...Ch. 11 - A eukaryote with a diploid number of 2n=6 carries...Ch. 11 - The accompanying chromosome diagram represents a...Ch. 11 - Suppose the genome of a bacterium contains a...Ch. 11 - DNaseI cuts DNA that is not directly associated...Ch. 11 - 10.17 Histone protein isolated from pea plants...Ch. 11 - 25. The molecular probes used in FISH can detect...Ch. 11 - Experimental evidence demonstrates that the...Ch. 11 - Prob. 27PCh. 11 - Genomic DNA from the nematode worm...Ch. 11 - What function do histone proteins perform in...Ch. 11 - Based on discussions of specific proteins and...
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- Describe the basic structure of a nucleosome. What is the role of histone H1?arrow_forwardWhat are the roles of the core histone proteins and of histone H1 inthe compaction of eukaryotic DNA?arrow_forwardAn article entitled “Nucleosome Positioning at the Replication Fork” states: “both the ‘old’ randomly segregated nucleosomes as well as the ‘new’ assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands” [Lucchini et al. (2002)]. Given this statement, how would one compare the distribution of nucleosomes and DNA in newly replicated chromatin? How could one experimentally test the distribution of nucleosomes on newly replicated chromosomes?arrow_forward
- How does chemical modification of the histones lead to changes in chromatin structure? Choose all that apply.A. They change the positions of the core histones indirectly.B. They change the positions of the core histones directly.C. They cause the histone N-terminal tails to become hyperextended.D. They act as binding sites for remodeling complexes.E. They change the affinity between the histone octamer and the DNA Please consider explaining both correct as well as incorrect optionsarrow_forwardUnlike linker DNA and deproteinized DNA, DNA segments wrapped around histone cores are relatively resistant to the hydrolytic actions of nucleases. Explain.arrow_forwardWhat is the role of the core histones in compaction ascompared to the role of histone H1?arrow_forward
- To hold bacterial DNA in a more compact configuration, specificproteins must bind to the DNA and stabilize its conformation.Several different proteins are involved in this process. Some of theseproteins, such as H-NS, have been referred as histone-like, due totheir functional similarity to the histone proteins found in eukaryotes.Based on your knowledge of eukaryotic histone proteins, whatbiochemical properties would you expect bacterial histone-likeproteins to have?arrow_forwardIn the first figure of “DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo Methylation of DNA,” the authors determined that the DNMT3L protein interacts with several histone proteins. Using Figure a and b (from that paper), explain how each of the 4 histones interact with DNMT3L.arrow_forwardYou are studying a small eukaryotic gene of about 2000 bp in length. About how many copies of histone H4 would you expect to find along this region of the chromosome?arrow_forward
- Why is transcriptionally active chromatin ∼10 times more susceptible to cleavage by DNase I than transcriptionally silent chromatin?arrow_forwardAssuming that 145 base pairs of DNA wrap around the histone octamer 1.75 times, estimate the radius of the histone octamer. Assume 3.4 Å per base pair and simplify the calculation by assuming that the wrapping is in two rather than three dimensions and neglecting the thickness of the DNA.arrow_forwardConsider the following sequence of DNA: 3'-TTA CGG-5'What dipeptide is formed from this DNA after transcription and translation? b. If a mutation converts CGG to CGT in DNA, what dipeptide is formed? c. If a mutation converts CGG to CCG in DNA, what dipeptide is formed? d. If a mutation converts CGG to AGG in DNA, what dipeptide is formed?arrow_forward
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