Campbell Essential Biology with Physiology (5th Edition)
Campbell Essential Biology with Physiology (5th Edition)
5th Edition
ISBN: 9780321967671
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece, Kelly A. Hogan
Publisher: PEARSON
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Chapter 11, Problem 4SQ

A eukaryotic gene was inserted into the DNA of a bacterium. The bacterium then transcribed this gene into mRNA and translated the mRNA into protein. The protein produced was useless and contained many more amino acids than the protein made by the eukaryotic cell. Why?

  1. a. The mRNA was not spliced as it is in eukaryotes.
  2. b. Eukaryotes and prokaryotes use different genetic codes.
  3. c. Repressor proteins interfered with transcription and translation.
  4. d. Ribosomes were not able to bind to tRNA.
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How is translation different in prokaryotes and eukaryotes? a. In prokaryotes, because they do not have a nucleus, the translation of mRNA occurs while it is being transcribed b. In prokaryotes, pre-mRNA translation before transcription occurs within the cell c.In prokaryotes, reverse trancriptase simultaneously translates and transcribes mRNAd.In prokaryotes, functional mRNA allows for translation to be skipped, and proteins are made during transcription
If eukaryotes have monocistronic genes, why is the number of known proteins more than the number of known genes?   A. Post-translational modification B. Alternative splicing C. Base substitution D. Post-transcriptional modification
What are two reasons that a eukaryotic gene (e.g. Green Fluorescent Protein in jellyfish) will not be expressed if it is inserted into a bacterial genome with no alterations? a. Bacterial genes do not contain introns b. Bacterial promoters have different consensus sequences than eukaryotic ones c. Transcription of this gene is affected by the simultaneous processes of transcription and translation d.
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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY