Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 11.1, Problem 3TQ
Summary Introduction
To review:
The lysis of E. coli cells lacking maltose porin by lambda phage.
Introduction:
The lambda phage infects Escherichia coli cells. It attaches to the host cell surface by binding to the maltose porin. This porin is responsible for the transport of maltose sugar into the bacterial cells. The maltose in the gut is provided by Bacteroides bacterial species that break down plant polysaccharides.
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Microbiology: An Evolving Science (Fourth Edition)
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- After a phage injects its DNA into a bacterial cell, the cell begins making proteins that make up the phage coat. Why does the same thing not happen when a generalized transducing particle injects the DNA it carries?arrow_forwardYou are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forwardA bacteriophage λ is found that is able to lysogenize itsE. coli host at 30°C but not at 42°C. What genes may bemutant in this phage?arrow_forward
- Which type of phages can perform generalized transduction?arrow_forwardIf a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?arrow_forwardIn an experiment, scientists grew bacteriophages in a culture with radioactive phosphorus (32P). The phages were then allowed to infect E. coli. a. The phages were then allowed to infect E. coli. Where was the 32P found after mixing with the E. coli?arrow_forward
- When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?arrow_forwardWhich type of phages can perform specialized transduction?arrow_forwardHow does Escherichia coli try to protect itself from phage attack,and how does T4 protect itself from these weapons?arrow_forward
- What is the purpose of including a tube in the phage assay in which only the E. coli culture is inoculated? Explain.arrow_forwardIn Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make? A. Protein does not enter the bacterium during transduction. B. DNA does not stay in the media after transduction. C. All of the phage DNA was radiolabeled. D. Bacteria can be transduced by phage.arrow_forwarda bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?arrow_forward
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