Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 16P
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a-e?
a. Mutation of consensus sequence in the lac promoter
b. Mutation of the repressor binding site on the operator sequence
c. Mutation of the lacI gene affecting the allosteric site of the protein
d. Mutation of the lacI gene affecting the DNA
e. Mutation of the CAP binding site of the lac promoter
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If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?
a)
There is a mutation in the repressor protein
b)
There is a mutation in the catabolite activator protein
c)
There is a mutation in the promoter region
d)
There is a mutation in the luxI protein
In the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the operator even in the absence of tryptophan____________.
a) the inducer cannot bind trpO, so operon gene transcription occurs
b) the active repressor cannot bind trpO, so operon gene transcription is attenuated
c)the active repressor binds tpO, so operon gene transcription is always repressed
d) the repressor binds the corepressor, and operon gene transcription occurs.
Gene expression in bacteria is controlled by the operon model.
a) Why is the operon model important?
b) Why is the trp operon known as a repressible operon?
Chapter 12 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 12 - 12.1 Bacterial genomes frequently contain groups...Ch. 12 - Transcriptional regulation of operon gene...Ch. 12 - Why is it essential that bacterial cells be able...Ch. 12 - Identify similarities and differences between an...Ch. 12 - The transcription of -galactosidase and permease...Ch. 12 - 12.6 Is attenuation the product of an allosteric...Ch. 12 - The trpL region contains four repeated DNA...Ch. 12 - The CAP binding site in the lac promoter is the...Ch. 12 - What role does cAMP play in transcription of lac...Ch. 12 - How would a cap- mutation that produces an...
Ch. 12 - Explain the circumstances under which attenuation...Ch. 12 - Consider the transcription of genes of the...Ch. 12 - Describe the lytic and lysogenic life cycles of ...Ch. 12 - 12.14 Define antisense RNA, and describe how it...Ch. 12 - 12.15 Attenuation of trp operon transcription is...Ch. 12 - 12.16 In the lac operon, what are the likely...Ch. 12 - Identify which of the following lac operon haploid...Ch. 12 - Prob. 18PCh. 12 - 12.19 List possible genotypes for lac operon...Ch. 12 - Suppose each of the genotypes you listed in parts...Ch. 12 - 12.21 Four independent mutants (mutants A to D)...Ch. 12 - Suppose the lac operon partial diploid...Ch. 12 - What is a riboswitch? Describe the riboswitch...Ch. 12 - 12.24 A repressible operon system, like the trp...Ch. 12 - 12.25 What is the likely effect of each of the...Ch. 12 - 12.26 Suppose that base substitution mutations...Ch. 12 - 12.27 Two different mutations affect. Mutant...Ch. 12 - How would mutations that inactivate each of the...Ch. 12 - The bacterial insertion sequence IS 10 uses...Ch. 12 - For an E. coli strain with the lac operongenotype...Ch. 12 - 12.31 How could antisense RNA be used as an...Ch. 12 - 12.32 Section describes the function of tRNA...Ch. 12 - The following hypothetical genotypes have genes A,...Ch. 12 - 12.34 Northern blot analysis is performed on...Ch. 12 - Prob. 35PCh. 12 - Prob. 36PCh. 12 - 12.37 The electrophoresis gel shown in part (a) is...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- There is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model, a. How many ribosome binding sites are there for the protein? b. How many promoters are there for the genes? c. How many start codons are there for the protein? d. How many RNA Polymerase binding locations are there for the genes? e. How many proteins will be fully functional? f. How many mRNA strands are made?arrow_forwardIf the above gene is one of the three structural genes of the lac operon that codes for the protein/ enzyme responsible for breaking lactose into two molecules of simple sugars, what triggers the activation of this gene? a. Absence of Inhibitory protein b. Presence of lactose c. Absence of lactose d. Presence of Inhibitory protein e. Absence of Regulatory proteinarrow_forwardIn the presence of tryptophan, and a mutation in the allosteric domain that abolishes tryptophan binding______. a) the active repressor cannot bind trp), so operon gene transription is attenuated b) the inactive repressor cannot bind trpO, so operon gene transcription occurs] c) the active repressor binds trpP, so operon gene trasncription is repressed, the inducer cannot bind trpO, so gene transcription occurs d) the repressor binds to the corepressor, and an operon gene transcription occursarrow_forward
- The gad operon is controlled by a number of transcription factors that regulate a promoter of approximately 450bp. Which would NOT explain why bacterial promoters are shorter than the typical eukaryotic promoter? a. Bacteria do not have nucleosomes b. Bacteria do not have mediator complex c. Each operon is regulated by only one signal d. All of the above are truearrow_forward. a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?arrow_forwardYou are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?arrow_forward
- If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is presentarrow_forwardOn its chromosome, an E. coli cell has a genotype of lacI− lacZ+ lacY+ lacA+. It has an F′ factor with a genotype of lacI+ lacZ+ lacY+ lacA+. What is the expected level of expression of the lac operon genes (lacZ+ lacY+ lacA+) in the absence of lactose?a. Both lac operons will be expressed.b. Neither lac operon will be expressed.c. Only the chromosomal lac operon will be expressed.d. Only the lac operon on the F′ factor will be expressed.arrow_forwardA mutation in the operator region of the trp operon can prevent the trp repressor from binding to this operator. When these mutant cells are placed in a solution containing chicken broth, which of the following happens? a. Tryptophan would bind to the repressor. b. The Repressor would bind to the operator irrespective of the presence of amino acids in the environment. c. The transcription of the trip operon would be inhibited. d. The transcription of the repressor protein is inhibitedarrow_forward
- Which of the following statements is false?a) A derivative of lactose serves as an inducer of the lac operon.b) Signal transduction provides a mechanism for a cell to sense the conditions of its external environment.c) Quorum sensing allows bacterial cells to sense the density of like cells.d) An example of a two-component regulatory system is the lactoseoperon, which is controlled by a repressor and an activator.e) An ORF is a stretch of DNA that may encode a protein.arrow_forwardWhat would occur if the repressor of an inducible operon weremutated so it could not bind the operator?(A) irreversible binding of the repressor to the promoter(B) reduced transcription of the operon’s genes(C) buildup of a substrate for the pathway controlled by theoperon(D) continuous transcription of the operon’s genesarrow_forwardWhat is the role of a substrate that controls a repressible operon? a. increase the production of inactive repressor proteins b. bind to the repressor protein and activate it c. bind to the repressor protein and deactivate it d. bind to the promoter region and decrease the affinity of RNA polymerase for the promoterarrow_forward
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