BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
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Chapter 12, Problem 17P
Interpretation Introduction
Interpretation:
The ambiguities of using the hydropathy plots to determine whether the protein is a membrane protein needs to be explained.
Concept introduction:
A graph that is named a hydropathy plot shows the location of an amino acid in a polypeptide and its hydrophobic/hydrophilic nature. This plot can be used to discover clusters of hydrophobic amino acids which show whether the specified polypeptide in the plot is a transmembrane protein or not.
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Roughly sketch the hydropathy plot for the given hypothetical membrane protein. Follow the color
assignment of the helical domains and properly label your plot and axes (NB: Only a rough sketch is
being asked. No need be exact with the hydropathy indices and residue numbers).
CO-
.A protein gives a single band on SDS gel electrophoresis, as shown
in lanes 1 and 2 below. There is little if any effect from adding
Molecular
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Chapter 12 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
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