Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them. a. Find the clone with the gene for lactase. b. Insert the plasmids into bacteria and grow the bacteria into clones. c. Isolate the gene for lactase. d. Create recombinant plasmids, including one that carries the gene for lactase.

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Answer 1

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Students have asked these similar questions

Choose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmid

(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.

If you were doing a cloning experiment where a plasmid was treated with PstI but the insert DNA did not have a PstI restriction site, what restriction enzyme(s) would be suitable to treat the DNA to be inserted?

Answer 2

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Answer 3

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the clone with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 8

Expert Solution & Answer

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To determine: The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction: Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer 12

Answer to Problem 1SQ

Correct answer: The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Answer 13

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed o reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 14

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