EBK CONCEPTS OF GENETICS
EBK CONCEPTS OF GENETICS
12th Edition
ISBN: 9780134818979
Author: Killian
Publisher: YUZU
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Chapter 12, Problem 24ESP

Following is a diagram of the general structure of the bacteriophage λ chromosome. Speculate on the mechanism by which it forms a closed ring upon infection of the host cell.

5 GGGCGGCGACCT—double-stranded region—3′

3′—double-stranded region—CCCGCCGCTGGA 5′

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Below is a depiction of a replication bubble. 5' AGCTCCGATCGCGTAACTTT 3' TCGAGGCTAGCGCATTGAAA CTAAAGCTTCGGGCATTATCG 3' GATTTCGAAGCCCGTAATAGC TATCGACS Consider the following primer which binds to the DNA replication bubble on the diagram above: 5'-GCUAUCG-3' Identify the DNA sequence to which this primer would bind and the orientation. If the replication fork moves to the right, will the primer be used to create the leading strand of replication or the lagging strand? Explain your answer b. If the replication fork moves to the left, will the primer be used to create the leading strand of replication or a. the lagging strand? Explain your answer. What would the next five nucleotides added to the primer by DNA polymerase? С.
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EBK CONCEPTS OF GENETICS

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