BIOCHEM-ACHIEVE(FIRST DAY DISCOUNTED)
9th Edition
ISBN: 2818000069358
Author: BERG
Publisher: MAC HIGHER
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Chapter 12, Problem 4P
Interpretation Introduction
Interpretation:
The diffusion coefficient at 
if protein in a membrane of 100 KDa has an effective viscosity of 1 poise and the average distance traveled by 100 kDa protein in 
and 
needs to be calculated.
Concept introduction:
Diffusion coefficient also called diffusivity is the diffusing quantity from one region to another passing through unit cross-section area per unit time. It is denoted by D.
Where,
K =Stefan Boltzmann constant 
T = Absolute temperature
viscosity of the solvent
r = radius of the sphere.
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7. Specificity of membrane transporters. A protein that transports amino acids across the cell membrane
was found to bind only a few amino acids efficiently. To find the specificity, many different amino acids and
substrate analogs were used as competitive inhibitors in transport studies at pH 5.9 with L-histidine (Km =
10 μM). The Ki values calculated from Lineweaver-Burk plots are shown in the table below. Comparing
the structures of L-histidine and the competitive inhibitors (for which most of them, you should know their
structures!), what can you conclude about the characteristics of molecules that this transport
protein binds at its active site?
K; (10-€ M)
Amino acid or
analog
L-Lys
L-Arg
Gly
L-Asp
D-His
Histamine
Dehydrourocanate
D-Arg
2
3
285
450
340
390
285
355
HN-
+
HN
+
Structure
-ΝΗ
-NH3
histamine
ΝΗ
-COO™
dehydrourocanate
10-¹1 M. A 1 nM (10-⁹ M) solution of lysozyme is
o An Fab fragment binds to lysozyme with a dissociation constant of Kå
treated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme be
bound to the Fab?
[F] =
nM
Multiple Choice. Imagine a solution containing only water, sodium, and glucose. If a voltage is applied to the solution, glucose would move:
(a) There would be no net movement of glucose
(b) In the opposite direction of sodium
(c) In the same direction and speed as sodium
(d) In the same direction as sodium, but slower.
(e) In the same direction as sodium, but faster.
Chapter 12 Solutions
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