Concept explainers
To review:
Binding and separation of mRNA (messenger ribonucleic acid) using the poly-dT (deoxythymine) column of ion exchange affinity chromatography. Binding of other RNA molecules like tRNA (Transfer RNA) and rRNA (Ribosomal RNA) to poly-Dt column.
Introduction:
Chromatography is defined as a method of separating large or small macromolecules or micro molecules entirely based on their physical and chemical properties. It is of the following types- Ion exchange chromatography, Affinity chromatography, Gel filtration chromatography, Paper chromatography, Column chromatography, Radial chromatography. Ion exchange affinity chromatography is used to separate mRNAs through their binding to the poly-Dt molecule of DNA (Deoxyribonucleic acid), which is linked to a column.
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Genetics: Analysis and Principles
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- The template strand (i.e.: the strand that is transcribed into RNA, which is usually represented “at the bottom”) of a segment of double helical DNA contains the sequence (5′) TCCGCTCCATCG (3′). What is the base sequence of the mRNA that can be transcribed from this strand?arrow_forwardKnowing that the genetic code is almost universal, a scientist uses molecular biological methods to insert the human - globin gene (shown in the figure below (Links to an external site.)) into bacterial cells, hoping the cells will express it and synthesize functional - globin protein. Instead, the protein produced is nonfunctional and is found to contain many fewer amino acids than does -globin made by a eukaryotic cell. Explain why and give thoughts as to how to overcome this.arrow_forwardConsider a stretch of DNA (a hypothetical gene) that has the sequence 5’ ATG-CTA-TCA-TGG-TTC-TAA 3’ A) Transcribe and translate this gene using the genetic code table. Be sure to label the mRNA 3’ and 5’ ends. Write the amino acid sequence using 1 letter abbreviations. B) Now, our hypothetical gene has undergone a mutation. The mutant sequence is....3’ TAC-GAT-AGT-ACC-AAT-ATT 5’5’ ATG-CTA-TCA-TGG-TTA-TAA 3’ Transcribe and translate the mutant sequence. Be sure to label the mRNA 3’ and 5’ ends. Write the amino acid sequence using 1 letter abbreviations. C) Indicate the type of mutation (nonsense, missense, silent, or frame shift) present. D) How severe of a consequence will this mutation likely be in terms of protein function (none, mild, moderate or severe)? Why?arrow_forward
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