Essentials of Genetics - Masteringgenetic
Essentials of Genetics - Masteringgenetic
9th Edition
ISBN: 9780134143699
Author: KLUG
Publisher: PEARSON
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Chapter 12, Problem 6PDQ
Summary Introduction

To review:

The coding experiments were done by using repeating copolymers to assign the different triplet codes to its correct amino acids.

Introduction:

Triplet codons are degenerate. It means more than one amino acid can be specified by more than one triplet codons. Wobble hypothesis was postulated by Crick in 1966. This hypothesis was used to predict that the initial two nucleotides of the codon are more critical as compared to the third nucleotide in attracting correct transfer RNA (ribonucleic acid) during the translation process.

Given:

AGG codes for arginine.

The table below shows the data of coding experiments carried out by using repeating copolymers.

Copolymer Codon produced Amino acids in a polypeptide
AG AGA, GAG arg (arginine), glu (glutamine)
AAG AGA, AAG, GAA Lys ( lysine), arg, glu

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Students have asked these similar questions
In a coding experiment using repeating copolymers , the following data were obtained: Copolymer Codons Produced Amino Acids in Polypeptide AG AGA, GAG Arg, Glu AAG AGA, AAG, GAA Lys, Arg, Glu AGG is known to code for arginine. Taking into account the wobble hypothesis, assign each of the four codons produced in the experiment to its correct amino acid.
The earliest work on the genetic code established UUU, CCC, and AAA as the codons for Phe, Pro, and Lys, respectively. Can you think of a reason why polyG was not used as a translation template in these experiments?
You start by looking at the mutations that Yanofsky recovered in TrpA. One of these mutations affected amino acid number 177 and changed it from Leucine to Arginine – because Yanofsky recovered it in his screen, that means that having an Arginine in this position does not allow the TrpA gene to function properly.  Assuming that this particular mutation induced by Yanofsky was a single nucleotide change, what are the possible codons of Leucine that could be found at this position in wild-type TrpA? What are the possible codons for Leucine that could be found in the mutant?. If you took this mutant E. Coli line (that has an Arginine at this location) and exposed it to a mutagen that could potentially change bases, what are the second mutations you would most likely discover that would restore the activity of the tryptophan synthetase gene and where would it be located?

Chapter 12 Solutions

Essentials of Genetics - Masteringgenetic

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