Prescott's Microbiology
11th Edition
ISBN: 9781260409062
Author: WILLEY, Joanne
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 13.3, Problem 3CC
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Describe the nature and functions of the following replication components and intermediates: DNA polymerases I and III, topoisomerase, DNA gyrase, helicase, single-stranded DNA binding proteins, Okazaki fragment, DNA ligase, leading strand, lagging strand, and primase.
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Chapter 13 Solutions
Prescott's Microbiology
Ch. 13.1 - MICRO INQUIRY Based on what we now know about...Ch. 13.1 - Prob. 2MICh. 13.1 - Retrieve, Infer, Apply 1. Briefly summarize the...Ch. 13.1 - Retrieve, Infer, Apply 2. Explain how protein was...Ch. 13.2 - MICRO INQUIRY To which carbon of ribose...Ch. 13.2 - MICRO INQUIRY How many H bonds are there between...Ch. 13.2 - Prob. 3MICh. 13.2 - Prob. 1CCCh. 13.2 - Retrieve, Infer, Apply What does it mean to say...Ch. 13.2 - Retrieve, Infer, Apply Amino acids are described...
Ch. 13.3 - MICRO INQUIRY What provides the energy to fuel...Ch. 13.3 - MICRO INQUIRY What is the difference between...Ch. 13.3 - MICRO INQUIRY Why cant DNA polymerase I perform...Ch. 13.3 - Retrieve, Infer, Apply How many replicons do...Ch. 13.3 - Prob. 2CCCh. 13.3 - Retrieve, Infer, Apply Describe the nature and...Ch. 13.3 - Retrieve, Infer, Apply Outline the steps Involved...Ch. 13.3 - Retrieve, Infer, Apply What is the end replication...Ch. 13.4 - Why is the nontemplate strand called the sense...Ch. 13.4 - Retrieve, Infer, Apply The coding region of a gene...Ch. 13.4 - Which strand of a gene has sequences that...Ch. 13.4 - Briefly discuss the general organization of tRNA...Ch. 13.5 - MICRO INQUIRY Are the -35 and -10 regions...Ch. 13.5 - Retrieve, Infer, Apply Outline the transcription...Ch. 13.5 - Retrieve, Infer, Apply What is a polycistronic...Ch. 13.5 - Retrieve, Infer, Apply What is a consensus...Ch. 13.5 - Tabulate the similarities and differences between...Ch. 13.6 - Prob. 1MICh. 13.6 - What is the difference between a codon and an...Ch. 13.6 - Prob. 2CCCh. 13.6 - Retrieve, Infer, Apply What is meant by code...Ch. 13.6 - Retrieve, Infer, Apply Is the genetic code truly...Ch. 13.7 - MICRO INQUIRY Why is simultaneous transcription...Ch. 13.7 - MICRO INQUIRY What would be the outcome if an...Ch. 13.7 - MICRO INQUIRY Why would it be impossible for...Ch. 13.7 - MICRO INQUIRY What provides the energy to fuel...Ch. 13.7 - Retrieve, Infer, Apply In which direction are...Ch. 13.7 - Retrieve, Infer, Apply Briefly describe the...Ch. 13.7 - Retrieve, Infer, Apply What are the translational...Ch. 13.7 - Prob. 4CCCh. 13.7 - Retrieve, Infer, Apply How many ATP and GTP...Ch. 13.8 - Prob. 1CCCh. 13.8 - Prob. 2CCCh. 13.8 - Retrieve, Infer, Apply Give the major...Ch. 13.8 - Prob. 4CCCh. 13 - Prob. 1RCCh. 13 - Prob. 2RCCh. 13 - Prob. 3RCCh. 13 - Prob. 4RCCh. 13 - Prob. 5RCCh. 13 - Streptomyces coelicolor has a linear chromosome....Ch. 13 - You have isolated several E. coli mutants: Mutant...Ch. 13 - Prob. 3ALCh. 13 - Prob. 4AL
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- Explain and list down the protocols in doing or preparation of materials for polymerase chain reaction.arrow_forwardHow does proofreading improve replication fidelity?arrow_forwardwhy does a higher agarose concentration render better resolution/separation of smaller DNA fragments? and what determines the distance of DNA fragments in gel electrophoresis?arrow_forward
-  Proofreads each nucleotide its template as soon as it is added to the growing strand. A) DNA Ligase B) Helicase C) DNA Polyerase D) Primase The genetic code A) has no redundancy but does have ambiguity B) has both redundancy and ambiguity C) has redundancy and not ambiguity D) has ambiguity E) has redundancyarrow_forwardAmplified target regions of four different samples were separated using gel electrophoresis. DNA fragments labeled with the isotope P32 were separated by gel electrophoresis. P32 was used to a) label fragments for imaging. b) distinguish between the fragment’s 3’ and 5’. c) observe the location of the fragments. d)speed up the rate of separation by electrophoresisarrow_forwardDescribe the proofreading activity of DNA polymerase.arrow_forward
- VISUAL SKILLS Compare Figure 20.7 with Figure 16.20.How does replication of DNA ends during PCR proceedwithout shortening the fragments each time?arrow_forwardHow did the ability to distinguish old and newly synthesized DNA strands enable Meselson and Stahl to verify that DNA replication is semiconservative?arrow_forwardExpand PCR? Describe the different Steps involved in this technique?arrow_forward
- How does NanoDrop quantify DNA?arrow_forward- Explain how you were able to identify plasmid in each sample using the gel, considering how linear DNA fragments of different length are separated by agarose gel electrophoresis.- Why do supercoiled, nicked and linear DNA sequences of the same size (kb) migrate at different rates during agarose gel electrophoresis?- How does SYBR Safe® enable the visualisation of the location of DNA fragments on the gel?arrow_forwardARE THEY TRUE OR FALSE? a) In a caesium chloride density gradient centrifugation method performed in the presence of ethidium bromide, supercoiled plasmid DNA binds more ethidium bromide than linear DNA. B)Tth DNA polymerase shows DNA-dependent DNA polymerase activity as well as RNA-dependent DNA polymerase activity. C)Magnesium ions stimulate polymerase activity. Therefore, it is better to use the highest concentration of magnesium in PCR amplification. D)When lambda bacteriophage infects E. coli cell lysis never occurs, and the infected bacterium can continue to grow and divide. E)The copy number refers to the number of molecules of an individual plasmid that are normally found in a single bacterial cell. F)RNase H selectively hydrolyzes phosphodiester bonds of RNA molecules in RNA:DNA duplexes.arrow_forward
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