Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134763040
Author: APPLING
Publisher: PEARSON
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Chapter 14, Problem 3P
Freshly prepared mitochondria were incubated with ß-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide. ß-hydroxybutyrate is oxidized by an NAD+ -dependent dehydrogenase. 
- The experimenter measured the rate of oxidation of ß-hydroxybutyrate and the rate of formation of ATP.
- Indicate the probable flow of electrons in this system.
- How many moles of ATP would you expect to be formed per mole of ß-hydroxybutyrate oxidized in this system?
- Why is ß-hydroxybutyrate added rather than NADH?
- What is the function of the cyanide?
- Write a balanced equation for the overall reaction occurring in this system (electron transport and ATP synthesis).
- Calculate the net standard free energy change (ΔGO') in this system, using
E'o values from Table 14.1 and a ΔGO' value for ATP hydrolysis of -32.2 kJ / mol.
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Freshly prepared mitochondria were incubated with B-hyroxybutyrate,
oxidized cytochrome c, ADP, P, and cyanide. B-Hyroxybutyrate is oxidized
by an NAD*-dependent dehydrogenase.
CoO
COO
CH,
CH,
H-C-OH
C=0
NAD+
NADH
CH,
+H+
CH,
The experimenter measured the rate of oxidation of B-hyroxybutyrate and
the rate of formation of ATP.
(a) Indicate the probable flow of electrons in this system.
(b) How many moles of ATP would you expect to be formed per mole of
B-hyroxybutyrate oxidized in this system?
(c) Why is B-hyroxybutyrate added rather than NADH?
(d) What is the function of the cyanide?
(e) Write a balanced equation for the overall reaction occurring in this
system (electron transport and ATP synthesis).
(f) Calculate the net standard free energy change (AG") in this system,
using E', values from Table 14.1 and a AG" value for ATP hydrolysis of
-32.2 kJ/mol.
When pure reduced cytochrome c is added to carefully prepared
mitochondria along with ADP, P, antimycin A, and oxygen, the
cytochrome c becomes oxidized, and ATP is formed, with a P/o
ratio approaching 1.0.
(a) Indicate the probable flow of electrons in this system.
(b) Why was antimycin A added?
(c) What does this experiment tell you about the location of cou-
pling sites for oxidative phosphorylation?
(d) Write a balanced equation for the overall reaction (including cyt
c oxidation and ATP synthesis).
A newly identified bacterium is unable to synthesize ubiquinone. A mobile electron carrier called CXC3 is used as a substitute. From the information provided in the table, calculate the delta G knot prime and the Keq value at 298 K for the redox reaction that occurs in this bacterium’s electron transport chain.
Explain the impact that using CXC 3 instead of ubiquinol will have on ATP production in the cell. How might this cell adapt to this situation?
Chapter 14 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 14 - Prob. 1PCh. 14 - When pure reduced cytochrome c is added to...Ch. 14 - Freshly prepared mitochondria were incubated with...Ch. 14 - Prob. 4PCh. 14 - Prob. 5PCh. 14 - Prob. 6PCh. 14 - Intramitochondrial ATP concentrations are about 5...Ch. 14 - Prob. 8PCh. 14 - Prob. 9PCh. 14 - Years ago there was interest in using uncouplers...
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