BIOLOGY:LIFE ON EARTH-W/ACCESS
11th Edition
ISBN: 9780134669076
Author: Audesirk
Publisher: PEARSON
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Chapter 14, Problem 4MC
Summary Introduction
Introduction:
Restriction enzymes or restriction endonucleases are enzymes that cleave deoxyribonucleic acid (DNA) at or near a particular recognition sequence. The cut may generate staggered ends or blunt ends. The restriction enzymes which generate staggered or ‘sticky’ ends are usually used in
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Students have asked these similar questions
a) what are restriction enzymes?
b) What is the main function of restriction enzymes in nature?
c) Compare and contrast the these enzymes in nature and in scientific research.
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel?
a. The 2000-bp fragment
b. The 1000-bp fragment
c. The 500-bp fragment
d. All will migrate equal distances.
Choose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)?
a.
Taq Polymerase was isolated from an organism found in Yellowstone Park
b.
Restriction enzymes leave sticky ends
c.
DNA sequencing allows us to read DNA sequences
d.
Restriction enzymes cut DNA at specific sites
e.
EcoRI and HindII are commonly used polymerases
Chapter 14 Solutions
BIOLOGY:LIFE ON EARTH-W/ACCESS
Ch. 14 - Which of the following is not true of a single...Ch. 14 - Imagine you are looking at a DNA profile that...Ch. 14 - 3. Which of the following is not a commonly used...Ch. 14 - Prob. 4MCCh. 14 - 5. DNA cloning is
a. making multiple genetically...Ch. 14 - Are organisms that contain DNA that has been...Ch. 14 - 2._________ is the process whereby bacteria pick...Ch. 14 - 3. The______ is a technique for multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FTB
Ch. 14 - Prob. 1RQCh. 14 - 2. What is a plasmid? How are plasmids involved in...Ch. 14 - 3. What is a restriction enzyme? How can...Ch. 14 - 4. Describe the polymerase chain reaction.
Ch. 14 - What is a short tandem repeat? How are short...Ch. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - How are DNA probes used to identify specific...Ch. 14 - Prob. 8RQCh. 14 - Describe several uses of genetic engineering in...Ch. 14 - Prob. 10RQCh. 14 - 1. As you may know, many insects have evolved...Ch. 14 - 2. All children born with X-linked SCID are boys....
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- A protein that can cut DNA at specific DNA base sequences is called aa. DNase. c. restriction enzyme.b. DNA ligase. d. DNA polymerase.arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardThe restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forward
- 1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequencesarrow_forward____ cut(s) DNA molecules at specific sites. a. DNA probes b. Restriction enzymes c. DNA polymerase d. DNA ligasearrow_forwardRestriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.arrow_forward
- Restriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forwardSome restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each strand. Sticky ends are useful in making recombinant DNA because Select one: a. Sticky ends contain the exact same nucleotides that allows fragments to splice together. b. Sticky ends contain nucleotides with complementary bases that allows fragments to splice together. c. Sticky ends contain the exact same nucleotides that can form hydrogen bonds. d. Sticky ends contain nucleotides with complementary bases that can form hydrogen bonds.arrow_forwardA restriction enzyme digests DNA into fragments.term the technique used to check the progression of this enzyme and separate DNA fragments.arrow_forward
- Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copiesarrow_forwardExplain how restriction enzymes recognize and digest DNA to create either blunt or sticky endsarrow_forwardIn 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.arrow_forward
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