Chapter 15, Problem 5Q

A.

Summary Introduction

To explain: What is observed in the budding of clathrin-coated vesicles from eukaryotic plasma membrane fragments when the adaptins are omitted.

Concept introduction:

Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.

B.

Summary Introduction

To explain: What would happen to budding clathrin-coated vesicles from eukaryotic plasma membrane fragments when the clathrin is omitted.

Concept introduction:

Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.

C.

Summary Introduction

To explain: What is observed in the budding of clathrin-coated vesicles from eukaryotic plasma membrane fragments when the dynamin are omitted.

Concept introduction:

Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.

D.

Summary Introduction

To explain: What is observed if the plasma membrane fragments were from a prokaryotic cell.

Concept introduction:

Vesicular transport is needed to shuttle the proteins in and out of an organelle. Coated vesicles are the vesicles that bud from the membrane which have a unique protein coat on their cytosolic surface. The vesicles that have an outer coat made of protein clathrin are called a clathrin-coated vesicle. If the adaptins, clathrin, and dynamic-GTP are added to the membrane preparation, the budding of the clathrin-coated vesicle from the eukaryotic plasma membrane fragments can be seen.