Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134145815
Author: KLUG
Publisher: PEARSON
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Textbook Question
Chapter 15, Problem 9PDQ
Explain why catabolite repression is used in regulating the lac operon and describe how it fine-tunes β-galactosidase synthesis.
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Explain why catabolite repression is used in regulating the lac operon and describe how it fine-tunes b-galactosidase synthesis.
Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.
Explain the role of CAP and cAMP in the regulation of the lac-operon.
Chapter 15 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 15 -
CASE STUDY | A mysterious muscular dystrophy
A...Ch. 15 -
CASE STUDY |A mysterious muscular dystrophy
A...Ch. 15 -
CASE STUDY |A mysterious muscular dystrophy
A...Ch. 15 -
HOW DO WE KNOW?
1. In this chapter, we have...Ch. 15 -
2. Review the Chapter Concepts list on p. 280....Ch. 15 - Describe which enzymes are required for lactose...Ch. 15 - Contrast positive versus negative regulation of...Ch. 15 -
5. Both attenuation and riboswitches rely on...Ch. 15 - For the lac genotypes shown in the accompanying...Ch. 15 -
7. For the genotypes and conditions (lactose...
Ch. 15 -
8. The locations of numerous lacI– and lacIs...Ch. 15 - Explain why catabolite repression is used in...Ch. 15 - Describe experiments that would confirm whether or...Ch. 15 - Predict the level of genetic activity of the lac...Ch. 15 - Predict the effect on the inducibility of the lac...Ch. 15 -
13. Describe the role of attenuation in the...Ch. 15 -
14. In a theoretical operon, genes A, B, C, and D...Ch. 15 - A bacterial operon is responsible for production...Ch. 15 - A marine bacterium is isolated and is shown to...Ch. 15 -
17. Why is gene regulation more complex in a...Ch. 15 -
18. List and define the levels of eukaryotic gene...Ch. 15 -
19. Distinguish between the cis-acting regulatory...Ch. 15 - Prob. 20PDQCh. 15 - Compare the control of gene regulation in...Ch. 15 - Many eukaryotic promoter regions contain CAAT...Ch. 15 -
23. What is RNA-induced gene silencing in...Ch. 15 - Although it is customary to consider...Ch. 15 - DNA methylation is commonly associated with a...Ch. 15 - The interphase nucleus appears to be a highly...Ch. 15 - It has been estimated that at least two-thirds of...
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- INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.arrow_forwardDescribe the regulation of the lac operon when lactose is present and glucose is absentarrow_forwardDescribe the role of the CRP–cAMP complex in positiveregulation of the lactose operon in E. coli.arrow_forward
- Describe the binding of RNA polymerase, repressors, andactivators to the lac operon when both lactose and glucose are scarce. What is the effect of these scarcities ontranscription of the lac operon?arrow_forwardDescribe the organization of the lac operon.arrow_forwardWhy it is essential to the positive stimulation of transcription of the lac operon ?arrow_forward
- Explain why (a) inactivation of the O2 or O3 sequence of the lac operon causes only a twofold loss in repression, and (b) inactivation of both O2 and O3 reduces repression ∼70-fold.arrow_forwardExplain how the data shown indicate that two operator sites are necessary for repression of the lac operon. What would the results have been if all three operator sites were required for the binding of lac repressor?arrow_forwardExplain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.arrow_forward
- describe the actions of the normal and mutated lac operon in absence of lactosearrow_forwardDescribe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite repression, functions. Use the lactose operon as an example.arrow_forwardMutation in the regulatory gene in the lac operon results in the formation of an inactive repressor. List and explain the effect of such a mutation on the expression of the structural gene for lactose in E.coli?arrow_forward
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