Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 17, Problem 18P
Interpretation Introduction
(a)
Interpretation:
The way in which dichloroacetate or DCA stimulates the pyruvate dehydrogenase activity.
Concept introduction:
Pyruvate dehydrogenase enzyme complex is required for the linking of glycolysis and TCA. It helps in the conversion of pyruvate into Acetyl CoA. Acetyl CoA then enters into the series of the citric acid cycle.
Interpretation Introduction
(a)
Interpretation:
If patients respond to DCA, then conclude the activity of pyruvate dehydrogenase.
Concept introduction: Pyruvate dehydrogenase enzyme complex is required for the linking of glycolysis and TCA. It converts the pyruvate into Acetyl CoA. Acetyl CoA then enters into the series of the citric acid cycle.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Closely related. Pyruvate dehydrogenase complex and a-ketoglutarate
a-ketoglutarate dehydrogenase complex are huge enzymes
consisting of three discrete enzymatic activities. Which amino acids
require a related enzyme complex, and what is the name of the
enzyme?
BIOCHEMISTRY. Could glycerol be used to regenerate the OAA for maintenance of TCA cycle activity? Yes or No? Explain.
Required partner. Aminotransferases require which of the following
cofactors:
a. NAD+/NADP+NAD+/NADP+
b. Pyridoxal phosphate
c. Thiamine pyrophosphate
d. Biopterin
Chapter 17 Solutions
Biochemistry
Ch. 17 - Prob. 1PCh. 17 - Prob. 2PCh. 17 - Prob. 3PCh. 17 - Prob. 4PCh. 17 - Prob. 5PCh. 17 - Prob. 6PCh. 17 - Prob. 7PCh. 17 - Prob. 8PCh. 17 - Prob. 9PCh. 17 - Prob. 10P
Ch. 17 - Prob. 11PCh. 17 - Prob. 12PCh. 17 - Prob. 13PCh. 17 - Prob. 14PCh. 17 - Prob. 15PCh. 17 - Prob. 16PCh. 17 - Prob. 17PCh. 17 - Prob. 18PCh. 17 - Prob. 19PCh. 17 - Prob. 20PCh. 17 - Prob. 21PCh. 17 - Prob. 22PCh. 17 - Prob. 23PCh. 17 - Prob. 24PCh. 17 - Prob. 25PCh. 17 - Prob. 26PCh. 17 - Prob. 27PCh. 17 - Prob. 28PCh. 17 - Prob. 29PCh. 17 - Prob. 30PCh. 17 - Prob. 31PCh. 17 - Prob. 32PCh. 17 - Prob. 33PCh. 17 - Prob. 34PCh. 17 - Prob. 35PCh. 17 - Prob. 36PCh. 17 - Prob. 37PCh. 17 - Prob. 38PCh. 17 - Prob. 39PCh. 17 - Prob. 40PCh. 17 - Prob. 41PCh. 17 - Prob. 42PCh. 17 - Prob. 43PCh. 17 - Prob. 44P
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Need help, please.arrow_forwardNeed help, please.arrow_forwardNeed help, please. In this scenario, a patient has beriberi. Beriberi patients can be treated through a thiamine-enriched diet. The patient was put on a thiamine-enriched diet but not all of their symptoms were alleviated. Through genetic testing, it was found that there was also a small defect in pyruvate dehydrogenase phosphatase that decreases its normal activity. 1. Explain why this genetic defect can cause beriberi symptoms (i.e. high pyruvate levels after a high-carb meal). 2. Why did thiamine supplementation not alleviate the symptoms for this patient?arrow_forward
- Long explanations are NOT NEEDED. ATP accounting. Consider 1 molecule of the sucrose (monomeric units: glucose and fructose) that will undergo complete oxidation. a. Number of pyruvate molecules after glycolysis.b. Net ATP produced in glycolysis only (via substrate-level phosphorylation).c. Number of NADH produced using the pyruvate dehydrogenase complex reaction.d. Number of NADH and FADH2 produced from Krebs cycle.e. Net ATP produced (complete oxidation via Malate aspartate shuttle).arrow_forward. Glucagon secretion causes inhibition of intracellular acetyl-CoA car- boxylase activity by several mechanisms. Name all you can think of.arrow_forwardHi, can someone help please. Thank you!arrow_forward
- disease. As such, a frontline treatment for Type 2 diabetes is the drug metformin, which acts indirectly to inhibit gluconeogenesis in the liver. You are a research biochemist who would like to develop new drugs that act to directly inhibit gluconeogenesis. You have just gained access to a library of thousands of small molecules of unknown activity, and you would like to identify lead compounds that have specific inhibitory activity against steps in the gluconeogenesis pathway. (a) into PEP in order to screen for inhibitors of enzymes specific to gluconeogenesis. Which enzymes do you need to purify, what cofactors and allosteric effectors do they require, and which reactants do you need to add to reconstitute the reactions for the first bypass? Which intermediates and products are generated? Your first approach is to reconstitute the initial set of bypass reactions that convert pyruvate (b) vitro reconstitution? What additional steps and enzymes are required in liver cells but are…arrow_forwardG. ENZYME CLASSIFICATION. Identify the main class of enzymes used to catalyzed the following reactions: 1. Lactate dehydrogenase: NADH+H NAD HC-OH CH3 CH Pynnte Lactate 2. Methylmalonyl-CoA mutase: CH CH SCOA CH,CH, SCOA coenzyme B12 COO COO methylmalonyl-CoA succinyl-CoA 3. Enolase: 0. H–Ċ–0–P–0- C-0–P-0- + H,0 HO–CH, CH 6 Phosphoenolpyruvate 2-Phosphoglycerate 4. Chymotrypsin: -0–CH,CH3 + H2O - RCOOH + HOCH,CH3 5. Pyruvate carboxylase: coo • co, • ATP + H,0 H-C-H . ADP + P, + 2H čoo CH, Pyruvate Oxaleacetatearrow_forward. Pyruvate can be processed under anaerobic conditions to ethanol (in yeast) or to lactate (in mammals), as shown. Explain the primary purpose of these reactions. Describe the major biochemical features of each reactionarrow_forward
- Long explanations are NOT NEEDED. I pretty much have an idea already about this lesson, I would just like to see if my answers are correct, if it's wrong then kindly correct and just add a short explanation. ATP accounting. Consider 1 molecule of the sucrose (monomeric units: glucose and fructose) that will undergo complete oxidation. a. Number of pyruvate molecules after glycolysis is 4.b. Net ATP produced in glycolysis only (via substrate-level phosphorylation) is 2.c. Number of NADH produced using the pyruvate dehydrogenase complex reaction is 1.d. Number of NADH and FADH2 produced from Krebs cycle is 12 and 4 respectively.e. Net ATP produced (complete oxidation via Malate aspartate shuttle) is 64.arrow_forwardLong explanations are not needed. Direct answers would suffice. a. Ribose-5-phosphate is produced by oxidative decarboxylation of 6-phosphogluconate catalyzed by the enzyme glucose-6-phosphate dehydrogenase. I. True II. False b. The pentose phosphate pathway occurs in the mitochondrion of tissues actively engaged in synthesis of fatty acids. I. True II. Falsearrow_forward27. Working at cross-purposes . Write a balanced equation showing the effect of simultaneous activation of glycogen phosphorylase and glycogen synthase. Include the reactions catalyzed by phosphoglucomutase and UDP-glucose pyrophosphorylase.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Anaerobic Respiration; Author: Bozeman Science;https://www.youtube.com/watch?v=cDC29iBxb3w;License: Standard YouTube License, CC-BY