Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 17, Problem 24P
Bacterial Pseudomonas species often possess plasmids encoding genes involved in the catabolism of organic compounds. You have discovered a strain that can metabolize crude oil and wish to identify the gene(s) responsible. Outline an experimental protocol to find the gene or genes required for crude oil
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Suppose that E. coli sustains a mutation in its gene for the lac operon repressor such that the repressor is ineffective. What effect would this have on the bacterium’s ability to catabolize lactose? Would the mutant strain have an advantage over wild-type cells? Explain your answer.
Refer to the diagram of pUC18 (Fig.) to determine which restrictionenzymes you could use to insert a gene that would not interfere with ampicillin resistance or production of β-galactosidase by the host cell.
Given the following phenotypes, explain how the mutation (identified by a (-) superscript) will affect the E. coli grown in lactose medium.
a. l+p+o+z-y+
b. i-p+o+z+y+
c. l+p+o-z+y+
d. l+p-o+z+y+
Chapter 17 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 17 - 15.1 What purpose do the bla and lacZ genes serve...Ch. 17 - The human genome is 3109 bp in length. How many...Ch. 17 - 15.3 Ligase catalyzes a reaction between the...Ch. 17 - You have constructed four different libraries: a...Ch. 17 - Using the genomic libraries in Problem 4, you wish...Ch. 17 - The human genome is 3109bp. You wish to design a...Ch. 17 - 15.7 Using animal models of human diseases can...Ch. 17 - 15.8 Compare methods for constructing homologous...Ch. 17 - 15.9 Chimeric genefusion products can be used for...Ch. 17 - 15.10 Why are diseases of the blood simpler...
Ch. 17 - Injection of double-stranded RNA can lead to gene...Ch. 17 - Compare and contrast methods for making transgenic...Ch. 17 - 15.13 It is often desirable to insert cDNAs into a...Ch. 17 - 15.14 A major advance in the s was the development...Ch. 17 - 15.15 The bacteriophage lambda genome can exist in...Ch. 17 - 15.16 The restriction enzymes Xho and Sal cut...Ch. 17 - 15.17 The bacteriophage has a single-stranded DNA...Ch. 17 - 15.20 You have identified a cDNA clone that...Ch. 17 - You have isolated a genomic clone with an EcoR I...Ch. 17 - 15.18 To further analyze the CRABS CLAW gene (see...Ch. 17 - 15.21 You have isolated another cDNA clone of the...Ch. 17 - 15.22 You have identified five genes in S....Ch. 17 - You have generated three transgenic lines of maize...Ch. 17 - 15.24 Bacterial Pseudomonas species often possess...Ch. 17 - 15.25 Two complaints about some transgenic plants...Ch. 17 - 15.26 In Drosophila, lossoffunction Ultrabithorax...Ch. 17 - Prob. 27PCh. 17 - The highlighted sequence shown below is the one...Ch. 17 - The RAS gene encodes a signaling protein that...Ch. 17 - Vitamin E is the name for a set of chemically...Ch. 17 - 15.31 You have cloned a gene for an enzyme that...
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- What makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants.arrow_forwardDescribe the experiment done by Frederick Griffith in 1928, where the non-lethal (rough) strain of Streptococcus pneumoniae bacteria killed experimental mice when mixed with the heat-killed smooth strain. Discuss what may have happened to the rough strain after the heat-killed smooth strain was introduced and why you think the rough strain killed the mice. What would be the conclusion and significance of this study and its finding?arrow_forwardGiven the following phenotypes, explain how the mutation (identified by a (-) superscript) will affect the E. coli grown in lactose medium. l+p+o+z-y+ i-p+o+z+y+ l+p+o-z+y+ l+p-o+z+y+arrow_forward
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