Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 17, Problem 14P
A major advance in the
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If you knew the mRNA sequence for the human insulin gene could you know what size cDNA fragment you would find on your DNA gel when you ran it against a size standard (a “molecular ruler”)? Would you continue with your insulin cloning experiment, if the DNA from your PCR was very different in size from that predicted by the insulin mRNA? Why or why not?
Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind to the DNA strands at a low temperature. PCR also preferentially amplify short segments of DNA. Would it be important to actually run the cDNA after the PCR on a DNA gel in order to check for a PCR product of the predicted size for the insulin gene? Why or why not?
As we described in class, in the early 1960's Francis Crick and colleagues set out to determine how many nucleotide bases make up a codon, before it was possible to sequence DNA and before Nirenberg and his colleagues solved the genetic code. To do this, they used a chemical mutagen that they knew made single nucleotide changes, used this mutagen to conduct a screen for mutations that disrupted a particular gene, and collected a number of different mutations in this gene. Briefly describe the logic they used to deduce that the codon length is 3 nucleotides long.
If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a plasmid, and transform it into E. coli, will I be able use the transformed E. coli to make the corresponding protein? Explain why, or why not? If you decide to do this, what would your cloning strategy be?
Chapter 17 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 17 - 15.1 What purpose do the bla and lacZ genes serve...Ch. 17 - The human genome is 3109 bp in length. How many...Ch. 17 - 15.3 Ligase catalyzes a reaction between the...Ch. 17 - You have constructed four different libraries: a...Ch. 17 - Using the genomic libraries in Problem 4, you wish...Ch. 17 - The human genome is 3109bp. You wish to design a...Ch. 17 - 15.7 Using animal models of human diseases can...Ch. 17 - 15.8 Compare methods for constructing homologous...Ch. 17 - 15.9 Chimeric genefusion products can be used for...Ch. 17 - 15.10 Why are diseases of the blood simpler...
Ch. 17 - Injection of double-stranded RNA can lead to gene...Ch. 17 - Compare and contrast methods for making transgenic...Ch. 17 - 15.13 It is often desirable to insert cDNAs into a...Ch. 17 - 15.14 A major advance in the s was the development...Ch. 17 - 15.15 The bacteriophage lambda genome can exist in...Ch. 17 - 15.16 The restriction enzymes Xho and Sal cut...Ch. 17 - 15.17 The bacteriophage has a single-stranded DNA...Ch. 17 - 15.20 You have identified a cDNA clone that...Ch. 17 - You have isolated a genomic clone with an EcoR I...Ch. 17 - 15.18 To further analyze the CRABS CLAW gene (see...Ch. 17 - 15.21 You have isolated another cDNA clone of the...Ch. 17 - 15.22 You have identified five genes in S....Ch. 17 - You have generated three transgenic lines of maize...Ch. 17 - 15.24 Bacterial Pseudomonas species often possess...Ch. 17 - 15.25 Two complaints about some transgenic plants...Ch. 17 - 15.26 In Drosophila, lossoffunction Ultrabithorax...Ch. 17 - Prob. 27PCh. 17 - The highlighted sequence shown below is the one...Ch. 17 - The RAS gene encodes a signaling protein that...Ch. 17 - Vitamin E is the name for a set of chemically...Ch. 17 - 15.31 You have cloned a gene for an enzyme that...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?arrow_forwardIn conventional PCR applications, a heat-resistant polymerase is used. Why? You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?arrow_forwardThe first publications describing the successful production and intracellular replication of recombinant DNA appeared in the early 1970’s. Since then, there is a long list of breakthroughs achieved by recombinant DNA technology. But which one of the following are NOT on that list? Select more than one answer ifneeded. a) Recombinant Factor VIII protein for the treatment of hemophiliacs b) Positional cloning of over 100 human disease genes c) Bacteria producing human insulin for the treatment of diabetic patients d) Reproductive cloning of over a dozen humansarrow_forward
- Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning allowing genes to be transferred from another biological species easily. Their work also gave rise to the development of different recombinant proteins with therapeutic applications like insulin and growth hormone. The former was cloned using Escherichia coli. coli in 1978. With this breakthrough, the first licensed drug produced using recombinant DNAtechnology was human insulin, developed by Genentech, licensed and marketed by Eli Lilly in 1982. Scientists were able to identify and isolate the gene fragment or the gene of interest, in this case, the gene that is responsible for producing insulin. Moreover, they were able to isolate the bacterial DNA of E. coli. The plasmid and DNA fragment were cut using a restriction enzyme. This DNA fragment was inserted into the plasmid using a DNA ligase. When the DNA fragment was then placed into the bacterial DNA, it was then introduced to the host cell (E. coli) and was then…arrow_forwardCRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. If this technology can change the future of Medicine, what specific benefits CRISPR can bring to genetic testing or analysis? How can CRISPR help to enhance gene therapy or treatment of genetic diseases?arrow_forwardYou are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?arrow_forward
- How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?arrow_forwardIn 1994, telomerase activity was discovered in human cancer cell lines. Although telomerase is not active in human somatic tissue, human somatic cells do contain the genes for telomerase proteins and telomerase RNA. Since inappropriate activation of telomerase may contribute to cancer, why do you think the genes coding for this enzyme have been maintained in the human genome throughout evolution? Are there any types of human body cells where telomerase activation would be advantageous or even necessary? Explain.arrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forward
- Although it is well known that X-rays cause mutations, they are routinely used to diagnose medical problems, including potential tumors, broken bones, and dental cavities. Why is this done? What precautions need to be taken?arrow_forwardWhat advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.arrow_forwardYou isolate genomic DNA from brain cells and heart cells and use PCR to amplify the promoter region of gene A, known to be methylated under certain circumstances. To determine which cell type has methylation in this region, you treat the DNA with sodium bisulfite, sequence the regions from both brain and heart cells, and compare to the untreated sequence, as shown below. Untreated: ATCCGGCGACG Brain: ATCCGGCGACG Heart: ATCTGGTGACG Given these results, which cell type would you expect to transcribe MORE "A" mRNA?arrow_forward
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