BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
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Chapter 17, Problem 39P
Interpretation Introduction
Interpretation:
The reason for the reaction of a symmetric molecule with an enzyme in an asymmetric way should be explained.
Concept introduction:
The asymmetric catalysis can be achieved via chiral reagents, asymmetric catalyst or via an enzyme that can induce asymmetry.
All the molecules present in biological systems are essentially single enantiomers. For example, all the amino acids are S- amino acids. Therefore, the biological system can serve as a chiral environment.
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6-25
substrate-band enzyme concentrations. The the
turnover number is equal to umax-
b)
V=Umax •57(Km+S)
anstont
For an enzyme that displays Michaelis-Menten kinetics, what is
the reaction velocity, V (as a percentage of Vmax), observed at
the following values?
a)
[S] = KM
C)
d)
e)
[S] = 0.5KM
[S] = = 0.1KM
[S] = 2KM
[S] = 10KM
w
reactores
-maximumrate of reaction
boteles conc.
Would you expect the structure of a competitive inhibitor of a
given enzyme to be similar to that of its substrate?
H.
OH
co
co2
но
H co,
1-isopropylmalate
2-isopropylmalate
Biosynthesis of leucine involves conversion of 1-isopropyimalate to 2-isopropylmalate (see above). This proceeds in four steps under basic
enzymic catalysis via an isolable compound produced in step 2. Write a detailed mechanism for this conversion. Then, draw the intermediate
compound) produced in step 2.
• You do not have to consider stereochemistry.
• Draw uninvolved carboxyl groups in the anionic state, and enolates as carbanions.
When needed, abbreviate CoenzymeA-S- as CH3S- In your drawing.
aale
Question #1: Choanoflagelletes are a unicellular ancestor to animals. One observation to support
this hypothesis is the appearance of adhesion molecules that are key to the development of
multiceullarity.
Bulk transport
Gap junctions
Animals.
Adhesion, cell
signaling
Single-celled
:}
Insects, mammals, and other animals
with bilateral symmetry (~10,000,000)
Jellyfish and their relatives (10,000)
} Sponges (10,000)
Choanoflagellates (150)
Despite their simple unicellular lifestyle they express adhesion molecules including cadherins
and lectins (King et al., 2003) but don't seem to have molecules that are typically found in the
extracellular matrix such as integrins or laminins (Williams et al., 2014).
Design a microscopy experiment to test the assertion that choanoflagellates have (some)
adhesion molecules and those molecules play a similar role in a closely related animal like
sponges.
Chapter 17 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
Ch. 17 - Prob. 1PCh. 17 - Prob. 2PCh. 17 - Prob. 3PCh. 17 - Prob. 4PCh. 17 - Prob. 5PCh. 17 - Prob. 6PCh. 17 - Prob. 7PCh. 17 - Prob. 8PCh. 17 - Prob. 9PCh. 17 - Prob. 10P
Ch. 17 - Prob. 11PCh. 17 - Prob. 12PCh. 17 - Prob. 13PCh. 17 - Prob. 14PCh. 17 - Prob. 15PCh. 17 - Prob. 16PCh. 17 - Prob. 17PCh. 17 - Prob. 18PCh. 17 - Prob. 19PCh. 17 - Prob. 20PCh. 17 - Prob. 21PCh. 17 - Prob. 22PCh. 17 - Prob. 23PCh. 17 - Prob. 24PCh. 17 - Prob. 25PCh. 17 - Prob. 26PCh. 17 - Prob. 27PCh. 17 - Prob. 28PCh. 17 - Prob. 29PCh. 17 - Prob. 30PCh. 17 - Prob. 31PCh. 17 - Prob. 32PCh. 17 - Prob. 33PCh. 17 - Prob. 34PCh. 17 - Prob. 35PCh. 17 - Prob. 36PCh. 17 - Prob. 37PCh. 17 - Prob. 38PCh. 17 - Prob. 39PCh. 17 - Prob. 40PCh. 17 - Prob. 41PCh. 17 - Prob. 42PCh. 17 - Prob. 43PCh. 17 - Prob. 44P
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