Concept explainers
a.
To determine:
The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).
Introduction:
The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.
b.
To determine:
Whether the genome of all mice should be integrated in a way which is described in the previous part.
Introduction:
A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.
c.
To determine:
The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.
Introduction:
The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.
d.
To determine:
The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.
Introduction:
The constructs of DNA (Deoxyribose
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ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
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