ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Textbook Question
Chapter 18, Problem 5P
The fly eyes shown in Fig. 18.7 are malformed because they lack a functional copy of a gene called fat facets that is required for eye development. The human genome encodes a protein called Usp9 that is similar in amino acid sequence to the fly Fat facets (Faf) protein. Likewise, the mouse genome encodes a protein called Fam that is similar to Faf and Usp9.
a. | How could you determine if human Usp9 can substitute for the Drosophila Faf protein? |
b. | How could you determine if, like Faf in flies, the mouse Fam protein is required for mouse eye development? |
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Lysine 4 of histone H3 (H3K4) is methylated in thenucleosomes of many transcriptionally active genes.Suppose you want to determine all the places in thehuman genome where nucleosomes contain methylated H3K4.a. Starting with an antibody that specifically bindsonly to the tails of histone H3s that have K4 methylation, what kind of experiment would you perform? Outline the major steps of this experiment.b. Do you think that you would get the same results ifyour starting material was skin cells in one experiment and blood precursor cells in a second experiment? Explain.c. Describe a follow-up experiment that could determine if your data from part (a) are consistent withthe idea that H3K4 methylation marks appear onlyat transcriptionally active genes
Chapter 18 Solutions
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...
Ch. 18 - The goal of the Knockout Mouse Project is to...Ch. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - a. Which genome manipulation technique would you...Ch. 18 - a. Diagram a knockin construct that could have...Ch. 18 - Prob. 16PCh. 18 - Prob. 17PCh. 18 - The transcription factor Pax6 is required...Ch. 18 - Mouse models for human genetic diseases are...Ch. 18 - One way to determine where inside a cell a protein...Ch. 18 - In Problem 5 in Chapter 17, you saw that a SNP...Ch. 18 - Scientists now routinely use CRISPR/Cas9 to make...Ch. 18 - Geneticists are currently considering using...Ch. 18 - a. Figures 18.9 and 18.12 demonstrated methods to...Ch. 18 - Nonhomologous end-joining NHEJ of a double-strand...Ch. 18 - One problem that researchers sometimes encounter...Ch. 18 - Researchers at the University of California at San...Ch. 18 - Prob. 28PCh. 18 - F. Port and S. Bullock at the University of...Ch. 18 - On Fig 18.14, locate the PAM site and identify the...Ch. 18 - Prob. 31PCh. 18 - Prob. 32PCh. 18 - Recall that Leber congenital amaurosis LCA, a form...Ch. 18 - One potential strategy for gene therapy to correct...Ch. 18 - Recently, scientists have used a mouse model for...
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