Lehninger Principles of Biochemistry (Instructor's)
Lehninger Principles of Biochemistry (Instructor's)
7th Edition
ISBN: 9781319117689
Author: nelson
Publisher: MAC HIGHER
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Chapter 18, Problem 2P
Summary Introduction

To explain: How the assay work in which the rate of alanine disappearance is equal to the rate of NADH disappearance.

Introduction:

An excess of pure lactate dehydrogenase and NADH in the reaction system are included to check the activity of the alanine amino transferase. The transfer of α-amino group to pyruvate is through glycolysis, and enzyme alanine transferase. The determination of the NADH molecules in the reaction is through this assay. The instrument used for measuring is UV visible spectrophotometer.

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