BIOLOGY:DYNAMIC SCIENCE-ACCESS >CUSTOM<
BIOLOGY:DYNAMIC SCIENCE-ACCESS >CUSTOM<
4th Edition
ISBN: 9781337254175
Author: Russell
Publisher: CENGAGE C
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Chapter 18, Problem 2TYK
Summary Introduction

Introduction:

The recombinant DNA is made by using two or more DNA fragments of different species or organisms. The fragments cleave by some specific bacterial enzymes, which are known as restriction endonucleases or restriction enzymes. These fragments are joined by the enzymes known as ligases.

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Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?
What is a cloning vector?   A. The DNA probe used to locate a particular gene in the genome.   B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.   C. The laboratory apparatus used to clone genes.         D.  An enzyme that cuts DNA into restriction fragments.
Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
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