BIOLOGY:DYNAMIC SCIENCE-ACCESS >CUSTOM<
4th Edition
ISBN: 9781337254175
Author: Russell
Publisher: CENGAGE C
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Chapter 18, Problem 2TYK
Summary Introduction
Introduction:
The recombinant DNA is made by using two or more DNA fragments of different species or organisms. The fragments cleave by some specific bacterial enzymes, which are known as restriction endonucleases or restriction enzymes. These fragments are joined by the enzymes known as ligases.
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Please answer these two questions regarding PCR:
a) Why do you need to perform PCR on DNA obtained from a crime scene?
b) Why so forensic labs analyze non-coding DNA rather than genes?
What is a cloning vector?
A.
The DNA probe used to locate a particular gene in the genome.
B.
An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.
C.
The laboratory apparatus used to clone genes.
D.
An enzyme that cuts DNA into restriction fragments.
Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
Chapter 18 Solutions
BIOLOGY:DYNAMIC SCIENCE-ACCESS >CUSTOM<
Ch. 18.1 - What features do restriction enzymes have in...Ch. 18.1 - Prob. 2SBCh. 18.1 - What information and materials are needed to...Ch. 18.2 - What is a transgenic organism?Ch. 18.2 - Prob. 2SBCh. 18.3 - What is a restriction fragment length polymorphism...Ch. 18.3 - Prob. 2SBCh. 18.3 - Prob. 3SBCh. 18 - Prob. 1TYKCh. 18 - Prob. 2TYK
Ch. 18 - Why are antibiotic resistance markers such as ampR...Ch. 18 - After a polymerase chain reaction (PCR), agarose...Ch. 18 - A cDNA and a cloned fragment of genomic DNA share...Ch. 18 - Prob. 6TYKCh. 18 - Which of the following is not true of somatic cell...Ch. 18 - Prob. 8TYKCh. 18 - Prob. 9TYKCh. 18 - Prob. 10TYKCh. 18 - Prob. 11TYKCh. 18 - Discuss Concepts A forensic scientist obtained a...Ch. 18 - 13. Suppose a biotechnology company has developed...Ch. 18 - Prob. 14TYKCh. 18 - You learned in the chapter that an STR locus is a...
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- Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forward. Explain how restriction enzymes work and why they are so important to recombinant DNA technology.arrow_forwardBriefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.arrow_forward
- Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.arrow_forwardExplain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.arrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardDescribe at least one important application of DNA technology in each of the following fields: medicine, DNA fingerprinting, and transgenic organisms.arrow_forwarda) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.arrow_forward
- Bioinformatics includes all of the following except (A) using computer programs to align DNA sequences. (B) using DNA technology to combine DNA from two different sources in a test tube. (C) developing computer-based tools for genome analysis. (D) using mathematical tools to make sense of biological systems.arrow_forwardWhich statement is true? a. There is no danger involved in recombinant DNA research in humans. b. Stringent safety rules make the use of recombinant DNA research impossible. c. There is no danger in releasing recombinant organisms into the environment. d. Stringent safety rules make the use of recombinant DNA research possible. e. There is no danger involved in recombinant DNA research in bacteria.arrow_forwardExplain the steps in recombinant DNA technology or rDNA technologyarrow_forward
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