Biological Science (7th Edition)
7th Edition
ISBN: 9780134678320
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Greg Podgorski, Emily Taylor, Jeff Carmichael
Publisher: PEARSON
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Textbook Question
Chapter 18, Problem 6TYU
Predict what would happen to regulation of the lac operon if the lad gene were moved 50,000
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To characterize the promoter of the gadA operon you made a series of deletion mutants removing pieces of
the promoter to see what would happen. The results are found below:
gad promoter
gada
gadX
gadz
450
+1
lacz activity
transcription
start site
pH 2.0
pH 7.0
A
gad promoter
beta-galactosidase (lacZ)
+++
450
gad promoter
beta galactosidase (lacZ)
+++
+++
300
+1
gad promoter
beta galactosidase (lacZ)
150
D
gad
beta galactosidase (lacz)
-450
150
E
gad promoter
beta-galactosidase (lacZ)
-450
-300
Based on these results, what can you conclude about the gad promoter?
O a. The promoter is only regulated by repression
Ob. The promoter is regulated by a mix of activation and repression
O c. The promoter is only regulated by activation
O d. The promoter has multiple operators and multiple enhancers
Illustrate the mechanism of the trp operon in E. coli.
Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.
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- In the regulation of the tryophan operon in bacteria, the co-occurence of transcription and translation plays a key role regulating full activation of operon expression. Diagram and explain how during the conditions of high tryptophan concentration this system regulates the expression of genes in the operon.arrow_forwardExamine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Question 1 Promoter Operator genC genB gen operon Regulator genA Transcription occurs when: A. The amino acid is not bound to the regulator, causing the regulator to bind to the operator. B. The amino acid is bound to the regulator, causing the regulator to bind to the operator. C. The amino acid is not bound to the regulator, causing the regulator to fail to bind to the operator. D. The amino acid is bound to the regulator, causing the regulator to fail to bind to the operator. E. The operon is…arrow_forwardExamine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genAarrow_forward
- Illustrate simplified region (map) of the lac operon genes. Identify the genes present in this region.arrow_forwardAnswer this 3 part question: (MC) What is the function of lactose in regulation of the lac operon? 1. activates a repressor protein 2. activates an activator protein 3. inactivates a repressor protein 4. inactivates an activator protein Consider a bacterial strain with a lac operon Р. О Y A a) Mention which parts of the DNA region shown in the diagram encode for • Enzyme proteins? • Operator • Promoter • Repressor gene b) Where would the lac repressor be bound in a E. coli cell that is growing in ( • high lactose? No lactosearrow_forwardPredict a possible phenotype from the following mutations in the Lac Operon: a deletion in “GGAATTGTGAGCGGATAACAATTTCAC.”arrow_forward
- Diagram the lactose operon in E. coli with the properorder of the elements lacI, lacO, lacY, and lacZ, and explainhow expression is controlled in the presence and in theabsence of lactose.arrow_forwardConsider the lac operon of E. coli. Specifically, explain the following: a. Describe the overall chromosomal structure/organization of the operon, indicating the location and function of the regulatory regions and the structural genes. b. Is lac a catabolic or anabolic pathway? Explain. Is the operon inducible or repressible? Explain. c. Describe the mechanisms involved in negative control & positive controlarrow_forwardWhat would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forward
- Explain how the lac operon is both positively and negativelycontrolled.arrow_forwardIn the: Mutation of the regulatory region for a repressor protein in the lac operon. Explain: (a) What is the process affected? (b) What is the Effect on the process? (c) Does it affect prokaryotes, eukaryotes or both?arrow_forwardSupply the words “off" or “on" in the blanks below: In an inducible operon, transcription is normally and must be turned In a repressible operon, transcription is normally and must be turned Briefly describe the overall function of the structural genes in the lac operon. Briefly describe the overall function of the structural genes in the trp operon. For the following descriptions of the bacterial lac operon, determine if lactose is present (P) or absent (A). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Allolactose is bound to the repressor For the following descriptions of the bacterial trp operon, determine if tryptophan levels are high (H) or low (L). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Tryptophan is bound to the repressor.arrow_forward
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