EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 18.5, Problem 3R
Summary Introduction
To review:
The concerns regarding the recombinant deoxyribonucleic acid (DNA) research that led to the moratorium after Cohen and Boyer’s experiment (they inserted viral DNA into bacterial DNA inventing recombinant DNA technology). Also, the reasons given by the scientists to assure the safety and lift the moratorium.
Introduction:
Many traits of the plants are altered according to the requirements by the DNA recombinant technology. There are many concerns raised against the introduction of genes in the genome of the plant via recombinant DNA technology.
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Answer all of the following questions
a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation
b) List two types of medical products that can be produced using DNA tech-
nology
c) Relate bioethics to the continued development of genetic engineering
techniques.
d) Describe two major goals of the Human Genome Project.
e) Distinguish between proteomics and genomics.
f) Outline the different applications of biotechnology
g) List some techniques of genetic engineering.
h) Delineate the transgenic organisms and their significance.
i) Explain the procedure for the following
PCR, Gel electrophpresis and Gene therapy
j) Describe human genome project and its main goals.
k) Discuss the drastic environmental impacts of oil spills and how the mi-
crobes like bacteria can help mitigate the problem.
l) Give an account of significance of transgenic organisms in food industry.
m) Describe the uses of DNA finger printing.
n) What is gene cloning?
o) Elaborately…
Dr. Lydia Villa-Komaroff...
O developed methods to use PCR to analyze crime scenes, and was the third Mexican-American woman in the
US to receive a doctorate degree in the sciences.
developed methods to generate recombinant insulin and was the third Mexican-American woman in the US
to receive a doctorate degree in the sciences.
O developed methods to sequence DNA, and was the third Mexican-American woman in the US to receive a
doctorate degree in the sciences.
O discovered restriction endonucleases, and was the third Mexican-American woman in the US to receive a
doctorate degree in the sciences.
The NIH Recombinant DNA Advisory Committee has what responsibility? a) Approving all forms of human clinical gene therapy b) Identifying all major risks to the human subjects in a specific research protocol c )Reviewing clinical trials involving human gene transfer d) Analyzing genomes and determining whether they appear mutagenic
Chapter 18 Solutions
EBK LIFE: THE SCIENCE OF BIOLOGY
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- Pick one (1) of the five (5) principles developed by the Ethics Committee for the Human Genome Organization that must be considered when the global community is confronted by new technology. Why do you feel that principle is MOST important.arrow_forwardWhy is using genomic DNA in gene therapy more efficacious?arrow_forwardWhat are some examples of ethical questions raised by the medicaluse of DNA technology?arrow_forward
- The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)arrow_forwardWhat are some safety and ethical concerns raised by the use of recombinant DNA technology? What are some dangers of introducing genetically engineered organisms into the environment?arrow_forwardFor what purposes is DNA extraction done? (give at least 3 purposes for which you may need to extract DNA)arrow_forward
- Today, it is easy to make transgenic plants and animals. What are some important safety and ethical issues raised by this use of recombinant DNA technology? Explain your answer.arrow_forwardPlease answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardThere are many PCR techniques available to suit the needs of all researchers in their laboratory task. (i) (ii) What is the major difference in the functions performed by the conventional PCR and real time PCR? Shania is planning to study the gene expression of Escherichia coli after a drug- treatment. She needs to decide between two types of chemistries to detect her PCR products (TaqMan Chemistry vs. SYBR Chemistry) using real-time PCR instruments. Compare and contrast between TaqMan Chemistry and SYBR Chemistry.arrow_forward
- It has been suggested that it would make the study of human diseases easier if cloned transgenic animals were produced that carried faulty versions of human genes (e.g., the gene that causes cystic fibrosis). a. Why would such animals be useful in medical research? : b. What ethical questions are raised by the creation of such transgenic animals?arrow_forwardWhat is the application of DNA extraction in healthcare? Cite at least 3 specific examples.arrow_forwardWhat is the main moralproblem about the cloning ofhuman individuals?arrow_forward
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