Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 19, Problem 12TYK
Summary Introduction
To review:
The concentration at which dideoxyribonucleotide produces a greater number of long complementary sequences.
Introduction:
DNA (deoxyribonucleic acid) sequencing is the method used to determine the sequences of the four
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Let’s assume the linker region of DNA averages 54 bp in length. How many molecules of H2A would you expect to find in a DNA sample that is 46,000 bp in length?
Give typing answer with explanation and conclusion to all parts
Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.
Describe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?
Chapter 19 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 19.1 - What additional biological questions can be...Ch. 19.2 - What is the principle behind whole-genome shotgun...Ch. 19.2 - Prob. 2SBCh. 19.2 - Prob. 3SBCh. 19.2 - Prob. 4SBCh. 19.3 - Prob. 1SBCh. 19.3 - Prob. 2SBCh. 19.3 - Prob. 3SBCh. 19.4 - Prob. 1SBCh. 19.4 - Prob. 2SB
Ch. 19 - Prob. 1TYKCh. 19 - How do pseudogenes differ from genes? a. They are...Ch. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - Prob. 5TYKCh. 19 - Prob. 6TYKCh. 19 - About 95% of the average human transcription unit...Ch. 19 - Prob. 8TYKCh. 19 - Prob. 9TYKCh. 19 - When two protein-coding genes have very similar...Ch. 19 - Prob. 11TYKCh. 19 - Prob. 12TYKCh. 19 - Prob. 13TYKCh. 19 - Discuss Concepts The genome of the yeast...Ch. 19 - Prob. 15TYKCh. 19 - Prob. 16TYKCh. 19 - Prob. 17TYKCh. 19 - Below is a sequence of 540 bases from a genome....
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- In chain termination sequencing, which of the following terminates the chain? a. The lack of a base at the 1' carbon b. The lack of OH at the 2' carbon c. The lack of OH at the 3' carbon d. The lack of phosphate at the 5' carbonarrow_forwardSuppose you have been directed to find new enzymes to use in the breakdown of wood in order to process biofuel (switchgrass, for example). Suppose you wanted to use fungal or bacterial DNA from the environment in order to do so. DNA can be unwound from the double stranded double helix into single strands, amplified, separated on gels by size, stained with dyes. It can be mutated by a variety of means. It can be sequenced. Describe one or more of the ways that you might manipulate DNA towards the stated goal. Relate the technology you plan to utilize to the structure of DNA. (You can break this into multiple posts, as multiple procedures might be used).arrow_forwardConsider a genome whose length is 1000 bp. "Shotgun" sequencing techniques are applied to the genome, resulting in 20 reads, with an average length of 50 bp. A very important point is that, even though 20×50 = 1000, there is no guarantee that ALL 1000 bp of the genome are represented in the fragments. Calculate the coverage. What does this value mean? Why would it be a good idea to have a coverage greater than 1?arrow_forward
- The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…arrow_forwardWhen performing Sanger sequencing a scientist accidentally forgot to include ddGTP in his sequencing reaction (he did include ddATP, ddCTP, and ddTTP along with all the other needed components). Which of the following statements are true? A.All DNA fragments will terminate at the first G base pair B.A color will be missing from the chromatogram. C.The sequencing primer will not contain G base pairs. D.There will be gaps in the chromatogram.arrow_forwardDNA solution is viscous because of the nature of chemical substance that can intercalate into the DNA helix. An example of such substance is acridine orange. experiments revealed that acridine orange causes an increase in the viscosity of DNA solution.how would you account for this effect?arrow_forward
- PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?arrow_forwardWhich of the following is true regarding next generation sequencing? 1. Next generation sequencing is also known as sequencing by synthesis 2. Next generation sequences uses DDNTPS to identify the base at the end of the nucleotide chain 3. Next generation sequencing will not work if chain terminating nuclear tides are included in the reaction 4. Next generation sequences uses fluorescently labeled ddNTPs instead of radioactivity labeled ddNTPs 5. Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same timearrow_forwardUse a drawing to illustrate the principle of DNA gel electrophoresis. Indicate roughly the comparative electrophoretic mobilities of DNAs with 150, 600, and 1200 bp.arrow_forward
- What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments? GelRed® moves down the agarose gel in response to the electric current and enables visualisation of the position of the nucleic acids within in the agarose gel. GelRed® intercalates with the Nucleic acid and, under UV light, fluoresces to enable visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the Nucleic acid and enables visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the amino acids in the agarose gel and enables visualisation of the position of their in the agarose gel.arrow_forwarda. Explain how the sea urchin and salmon data demonstrate both of Chargaff’s rules b. How significant is the Chargaff’s rule in the creation of the DNA model? c. You are a molecular biologist and you observed that the amount of the combined purinenitrogenous base in the DNA of an unknown species of freshwater fish in your vicinity is equivalentto 68%, and you determined that it possesses 16% Thymine in its RNA. How much Adenine,Guanine and Cytosine is present in the DNA of this freshwater fish? Explain how you arrived at youranswer.arrow_forwardSanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different lengths obtained by adding: All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP), together with all of the 4 deoxynucleotides (dATP, dGTP, dCTP and dTTP), to all of the reaction vials All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; together with one of the 4 deoxynucleotides (dATP, dGTP, dCTP and dTTP), one for each lane, in each vial. One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane, together with all of the 4 deoxynucleotides (dATP, dGTP, dCTP and dTTP) in each vial One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lanearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
An Introduction to the Human Genome | HMX Genetics; Author: Harvard University;https://www.youtube.com/watch?v=jEJp7B6u_dY;License: Standard Youtube License