PRESCOTT'S MICROBIO W/PROCTORIO
11th Edition
ISBN: 9781264731060
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 19, Problem 1AL
Consider the fact that the use of 16S rRNA sequencing as a taxonomic and phylogenetic tool has resulted in tripling the number of bacterial phyla. Why has the advent of this genetic technique expanded the currently accepted number of microbial phyla?
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Let's say that you're a scientist trying to analyze newly sequenced bacterial DNA genomes from
an environmental sample of rainforest soil, and your first task is to organize all of the genomes
you find into a phylogenetic tree. If you couldn't use the 16S rRNA gene sequence to create the
tree, which of these alternative genes would work best for your task of creating a tree that works
for all the bacteria in your sample?
The gene for Bacteriochlorophyll A
A gene for an RNA polymerase component protein
The first gene in the histidine biosynthesis pathway
The first gene in the LPS synthesis pathway
select the correct options:
A) For organisms that diverged >74 mya, ignore 3rd base positions within codons because they will offer little phylogenetic information for organisms.
B) use different parts ofthe genome to estimate different parts of a phylogeny
C) Use only 1st base positions within codons because they increase rapidly at first, then level off as result of single subsitituions at the same sites
D) Use slowly evolvong sites in the squecne, such as 2nd base positions within codonsfor analyzing the oldest relationships among organisms.
Give typing answer with explanation and conclusion
In thinking about the clinical application of phylogenetics, discuss the role of phylogenetics in these areas.
a) Predictions of drug resistance
b) Identifying the origin of an outbreak
c)Predicting the spread of a disease
Chapter 19 Solutions
PRESCOTT'S MICROBIO W/PROCTORIO
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - Prob. 2CCCh. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 3CCCh. 19.4 - Could a phylotype be considered an OTU? What about...
Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3CCCh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4CCCh. 19.6 - Prob. 1CCCh. 19.6 - Describe two different situations in which it...Ch. 19 - Prob. 1RCCh. 19 - Prob. 2RCCh. 19 - Prob. 3RCCh. 19 - Prob. 4RCCh. 19 - Prob. 5RCCh. 19 - Prob. 6RCCh. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 3ALCh. 19 - Prob. 4ALCh. 19 - Prob. 5ALCh. 19 - Prob. 6ALCh. 19 - Prob. 7AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- List three reasons why rRNA genes are suitable for phylogeneticanalyses.arrow_forwardYou have been tasked with designing a new computer algorithm to identify protein-coding genes in bacterial genomes.a) Name 3 specific criteria/patterns you will use to define whether a given sequence could contain a full-length protein-coding gene, and briefly justify your choices.b) Do you think your algorithm will work well to detect proteins encoded by the archaean Sulfolobus acidocaldarius? Why or why not?c) Do you think your algorithm will work well to detect proteins encoded in the eukaryotic yeast Saccharomyces cerevisiae? Why or why not?arrow_forwardExplain why the answer is correct. For eukaryote cells to evolve into new types of eukaryotic organisms, which of the following would one expect to occur? The correct answer is E a) these cells have evolved because they have different DNA because of mutations b) Their endoplasmic reticulum is synthesizing new and/or different proteins. c) These specialized cells activate new genes to synthesize new proteins. d) these organisms have new characteristics because they have acquired new genes. e) all of these are truearrow_forward
- The fact that a prokaryote has the ability to make a new protein that is placed in the cell membrane of the mesosome is important to the process of evolution because this represents a) the mutation of a gene, b) new DNA producing a new genetic variation, c) r RNA using a new type of m RNA, d) the species having a greater degree of genetic variability, e) all are truearrow_forwardfind an example of a set of genes that have been horizontally gene transferred between bacteria or archaea. Which organisms are the genes transferred between? Explain the set of genes that were transferred. Include what they are for and what the advantage to sharing those genes are.What do the organisms use the genes for?Which organisms the transfer happened between?What might be the advantage be to share the genes with other organisms?arrow_forwardGenes of different species but possessing a clear sequence and functional relationship to each other area) Orthologb) Syntenyc) Paralogd) Microarrayarrow_forward
- You isolate bacteria from several different pools at the recreation center. Curious about which bacteria likely have a common ancestor with your reference pool (the Buffalo-pool), you BLAST the 16S ribosomal gene from the Buffalo pool bacteria against the 16S ribosomal genes from the other pools. The results are shown below. Which of the bacteria likely have a common ancestor with the Buffalo-pool bacteria? Use e as a cutoff for homology. Organism Dive-well bacteria Competition-pool bacterial Practice-pool bacteria Puddle bacteria Puddle bacteria Practice-pool bacteria Competition-pool bacteria Dive-well bacteria E-value 9e-175 -300 8e 4e-2 4arrow_forwarda) What is a phylogenetic tree? b) What is a branch point? c) What are sister taxa? d) What is a ‘rooted’ phylogenetic tree?arrow_forwardThe hypercycle model explains which of the following? A) How the evolutionary transition from prokaryotic to eukaryotic cells through acquisiton of membrane bound organelles. B) How viruses first originated alongside cellular life through a hyper replicating RNA lineage. C) How individual replicating molecules like RNA, DNA, and proteins entered a molecular mutualism and became encapsulated forming protocells. D) How cycles of heat and electricity experimentally lead to the formation of amino acids in the lab.arrow_forward
- Describe the impact of lateral gene transfer on bacterial genome evolution.arrow_forwardImagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.arrow_forwardIf two otherwise dissimilar organisms share similar genomic DNA, to what would you attribute the genomic similarity? A)DNA is not a reliable basis for phylogenetic decisions. B)The shared genes have evolved a number of times and have no phylogenetic significance. C)The shared genes are ancient and probably no longer function within the altered physiological states of modern organisms. D)The shared genes developed early in evolution and are likely to be applicable to a wide diversity of organisms. E)The two taxa are more closely related than formerly believed because they may share a common ancestorarrow_forward
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license