CAMPBELL BIOLOGY W/ACCESSCODE
CAMPBELL BIOLOGY W/ACCESSCODE
11th Edition
ISBN: 9781323708217
Author: Urry
Publisher: PEARSON C
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Chapter 19, Problem 4TYU
Summary Introduction

Introduction: Bacteria are single-celled prokaryotic microorganisms. A bacteriophage is also called as bacterial virus that is formed when a virus infects a bacterium. T2 and T4 are enterobacteria phages that infect and kill E. coli. They are also known as T-even phages because they are double stranded. T-even phages are commonly used as model organisms for various genetic studies. The experiments performed by Hershey and Chase using bacteriophages already proved that DNA is the genetic material of inheritance.

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If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license