Biology - Study Guide
8th Edition
ISBN: 9780321501561
Author: Martha R. Taylor
Publisher: PEARSON EDUCATION (COLLEGE)
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Chapter 20, Problem 10TYK
Summary Introduction
Introduction: Recombinant deoxyribonucleic acid (DNA) technology is the process of combining the DNA sequence of two or more organisms artificially to acquire the desired protein product. The process is brought about by inserting the gene of interest into a plasmid called recombinant plasmid. This recombinant plasmid is then introduced into a bacterium to produce a clone of cells with the gene of interest.
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Students have asked these similar questions
Which step follows the assembly of new DNA strands by DNA polymerase?
A. Enzymes unwind and separate the two strands of DNA.
B. Repair enzymes correct potential mutations in the DNA sequence.
C. Primers base-pair with the exposed single DNA strands.
D. DNA ligase seals any gaps remaining between bases of new DNA.
Which step follows the assembly of new DNA strands by DNA polymerase?
a.DNA ligase seals any gaps remaining between bases of new DNA.
b.Primers base-pair with the exposed single DNA strands.
c.Repair enzymes correct potential mutations in the DNA sequence.
d.Enzymes unwind and separate the two strands of DNA.
What do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together?
a.) an amino acid sequence
b.) DNA ligase
c.) restriction enzymes
d.) mRNA
Chapter 20 Solutions
Biology - Study Guide
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- Which is true of the lagging strand in DNA synthesis? a. It is built elongating towards the replication fork b. It is composed entirely of RNA c. It is built in the 3’ to 5’ direction d. It elongates in a series of segments, rather than continuouslyarrow_forwardWhich technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro? a. The CRISPR-Cas9 system b. PCR-based random mutagenesis c. PCR-based site-specific mutagenesis d. RT-PCRarrow_forwardWhich helps prevent errors in DNA replication? A) Complementary base pairing reduce errors B) DNA ligase checks the DNA for errors C) DNA is located in the ribosomes D) Any base can pair with any other basearrow_forward
- Which statement is true of reverse transcriptase? a. It is a nucleic acid. b. It infects cells. c. It transcribes RNA to make DNA. d. It is a lipid.arrow_forwardChoose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forwardWrite short answers. i. RNA not exists in double stranded from as DNA. Why?arrow_forward
- Which describes the role of primase during replication? a. It catalyzes the formation of phosphodiester bonds using NTPs as substrates. \ b. It coordinates synthesis of the leading strand and the lagging strand. c. It functions as a holoenzyme that polymerizes in the 3’→ 5’ direction. d. It uses an exonuclease activity to remove incorrect nucleotides.arrow_forwardThe structure of DNA revealed how information is stored in the base sequence along a DNA strand. But what information is stored and how is it expressed?arrow_forwardGive only typing answer with explanation and conclusion Briefly describe how DNA is able to be visualized by UV light. Use words like direct absorbance of DNA)arrow_forward
- (short answer) Imagine you’ve been offered a deal from a genomics company. You can get a free genome sequence – an analysis of all of your DNA that includes a report of your ancestry, traits and a medical profile. The medical profile tells you about diseases for which you have a low risk of getting, and also those you have a high risk of getting. Are you interested? Why or why not?arrow_forwardWhat is the role of Proteinase K in DNA isolation? A. Inhibit enzymes B Catalyze breakdown of nuclei acids C Degrade proteins D Remove disultide bongs in proteinsarrow_forwardevaluate the safeguards used in research using genetic engineering.arrow_forward
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