Biology - Study Guide
8th Edition
ISBN: 9780321501561
Author: Martha R. Taylor
Publisher: PEARSON EDUCATION (COLLEGE)
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Chapter 20, Problem 8TYK
Summary Introduction
Introduction: Complementary deoxyribonucleic acid (cDNA) is a DNA copy of mRNA synthesized from a single-stranded RNA template in a reaction catalyzed by the reverse transcriptase enzyme. The sequence of cDNA is complementary to its template RNA sequence.
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Students have asked these similar questions
Part A:
During cloning, the DNA is cut with a restriction enzyme giving it what?
A.
more introns
B.
sticky ends
C.
a polyA tail
D.
a binding site for a transcription factor
Part B:
If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use?
A.
PCR
B.
epigenetics
C.
hybridization
D.
gel electrophoresis
Part C:
If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible?
A.
transcription factor
B.
rRNA
C.
ribosome
D.
RNA polymerase
Part D:
If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what?
A.
PCR
B.
gene therapy
C.
stem cell work
D.
DNA fingerprinting
A. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa?
C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?
A biology professor at a prestigious university stated to his introductory
class that "Today, a basic understanding of DNA and genetics is as important as a basic understanding of computers and the internet."Based upon what you have learned about DNA and gene expression in this module, how could you make a case to support this statement?
Chapter 20 Solutions
Biology - Study Guide
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- A scientist splices a eukaryotic promoter in front of a bacterial gene and inserts the gene in a bacterial chromosome. Would you expect the bacteria to transcribe the gene?arrow_forwardA student wants to observe how the environment of bacteria affects gene expression. How should the experiment be designed? A. exposing the same species of bacteria to the same carbohydrates in each sample B. exposing the same species of bacteria to different carbohydrates in each sample C. exposing different species of bacteria to the same carbohydrates in each sample D. exposing different species of bacteria to different carbohydrates in each samplearrow_forwardIf you made a cDNA library from human brain cells and one from human liver cells would the clones in your two libraries be the same? a)Yes, the libraries would be identical. b)You cannot make cDNA libraries from these cell types. c) No, the libraries would have few overlapping clones. d) The libraries would have a number of similar clones for common cellular proteins but would have a number of unique clones associated specifically with each cell type.arrow_forward
- With regard to transcriptional termination in eukaryotes, which model suggests that RNA polymerase is physically removed from the DNA? a. Allosteric model b. Torpedo model c. Both models d. Neither modelarrow_forwardSickle cell anemia is a disease caused by a mutation at the genotypic level. A person with two copies of the gene has the disease, but a person with one copy of the gene does not have the disease. The diagram shows how this mutation affects an organism. Use the image to answer the question. Which conclusion can you draw based on the diagram? A. The mutation changed the amino acid sequence during the translation step. B. A mutation caused the information in DNA to change during the transcription step. C. A change in the DNA sequence can be carried over to the translation and transcription steps. D. The change that affected the translation and transcription steps does not affect the organism.arrow_forwardf you made a change in the promoter sequence in the DNA that inactivates the promoter, what would happen at the RNA level? A-Nothing, because the RNA would be made as usual B-Transcription factors would be unable to bind and the RNA polymerase would not be recruited to the DNA, so no RNA would be made. C-The mutation of the DNA would be carried through to the RNA sequence. D-The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made. EXPLAIN WHY THE ANSWER YOU CHOOSE IS CORRECTarrow_forward
- (a) Why can there be multiple codons for an amino acid? Why would this have evolved? (b) What is the advantage of Illumina Next Generation Sequencing?arrow_forwardDesign an experiment, using western blot, to test whether a certain amino acid sequence sends a protein to the ER. Draw what the results of that blot would look like if the sequence sends the protein to the ER and what it would look like if it does not.arrow_forwardWhat strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cellsarrow_forward
- a) What is a gene promoter? b) What proteins bind to a promoter? c) What do those proteins do to control gene expression?arrow_forwardBase analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.arrow_forwardThe original DNA base sequence is 5’-AGCGTTACCGT-3’; a mutation in the DNA strand results in the base sequence 5’-AGGCGTTACCGT-3’. What can you conclude about the mutation? A. It is a frameshift mutation. B. It is a silent mutation. C. It is a deleterious mutation. D. It may result in a single amino acid change in the protein being coded for by this base sequence.arrow_forward
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