Biology - Study Guide
8th Edition
ISBN: 9780321501561
Author: Martha R. Taylor
Publisher: PEARSON EDUCATION (COLLEGE)
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Textbook Question
Chapter 20, Problem 6TYK
The following segment of DNA has restriction sites I and II, which create restriction fragments a, b, and c. Which of the gels produced by electrophoresis would represent the separation and identity of these fragments?
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Chapter 20 Solutions
Biology - Study Guide
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- How would the results of this activity have been different if the DNA sequence you digested were circular instead of linear? When restriction digests are performed and analyzed in the laboratory, one lane of the agarose gel is loaded with DNA ladders, which are DNA fragments of known sizes. What is the point of including these size standards?arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, the smallest fragments between DNA sequence A and B were how many base pairs?arrow_forwardA 22-kb piece of DNA has the following restriction sites:A batch of this DNA is first fully digested by HpaI alone, then another batch is fully digested by HindIII alone, and finally, a third batch is fully digested by both HpaI and HindIII together. The fragments resulting from each of the three digestions are placed in separate wells of an agarose gel, separated by gel electrophoresis, and stained by ethidium bromide. Draw the bands as they would appear on the gel.arrow_forward
- When linear DNA is sequenced, the nucleotide base pairs are numbered from the start to finish. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?arrow_forwardthe restriction enzyme NotI recognizes the following sequence : 5'-GCGGCCGC-3' . On average, how oftern should this enzyme cleave DNA?arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, which DNA sequence held the largest size fragment? DNA sequence A or B? arrow_forward
- If a 1000 bp of DNA were inserted between the two restriction sites, how would the banding pattern on the gel differ from the one you drew in part a? (PART A WITH THE FIRST PART OF THE QUESTION IS ATTACHED)arrow_forwardAbove are the results of gel electrophoresis following digestion with restriction enzymes. What is the total length of the DNA fragment? Which enzyme, HindIII or EcoRI, produced a larger fragment? What part of DNA causes it to be negatively charged in order for electrophoresis to work?arrow_forwardWhen circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed position on the DNA, all the way around, usually in a clockwise manner. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?arrow_forward
- If a restriction enzyme cuts at this DNA sequence: TACGGAT, in general what is this sequence called?arrow_forwardA molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is 62% G + C. How many times, on average, are restriction sites for the following restriction enzymes likely to be present in this DNA molecule? a. HindIII (recognition sequence is AAGCTT)arrow_forwardIf the GAATTC palindrome repeats are randomly found along the DNA strand, then what can you say about the sizes of the fragments that will be produced when the DNA is digested with a restriction enzyme that recognizes that sequence? How does the total length of the fragments relate to the size of the original DNA fragment?arrow_forward
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