Biology - Study Guide
8th Edition
ISBN: 9780321501561
Author: Martha R. Taylor
Publisher: PEARSON EDUCATION (COLLEGE)
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Chapter 20, Problem 13TYK
Summary Introduction
Introduction: Recombinant DNA technology involves the production of the desired gene by combining the genetic material of two or more organisms. It is a wide application in the field of agriculture, where rDNA is introduced to the target plant cell with the help of vectors injected into suitable bacterial cells.
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Plasmids are important in biotechnology because they are
Group of answer choices
a. a vehicle for the insertion of foreign genes into bacteria
b. recognition sites on recombinant DNA strands
c. surfaces for protein synthesis in eukaryotic recombinants
d. viruses incorporated into the host DNA
Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease
b. virus receptors
c. a gene for antibiotic resistance
d. reverse transcriptase
When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
Chapter 20 Solutions
Biology - Study Guide
Ch. 20 - In what ways would third-generation sequencing be...Ch. 20 - The following schematic diagram depicts an...Ch. 20 - Which of the following DNA sequences would most...Ch. 20 - a. When PCR is used to prepare a DNA fragment for...Ch. 20 - a. What are some of the benefits of determining...Ch. 20 - Prob. 6IQCh. 20 - What are some of the practical and ethical...Ch. 20 - Prob. 8IQCh. 20 - Prob. 1SYKCh. 20 - Fill in the table on the previous page on the...
Ch. 20 - Prob. 3SYKCh. 20 - Prob. 1TYKCh. 20 - Prob. 2TYKCh. 20 - Gel electrophoresis is a means of separating...Ch. 20 - Prob. 4TYKCh. 20 - Prob. 5TYKCh. 20 - The following segment of DNA has restriction sites...Ch. 20 - Prob. 7TYKCh. 20 - Prob. 8TYKCh. 20 - Prob. 9TYKCh. 20 - Prob. 10TYKCh. 20 - Prob. 11TYKCh. 20 - Which enzyme is used in the polymerase chain...Ch. 20 - Prob. 13TYKCh. 20 - STRs (short tandem repeats) are a valuable tool...Ch. 20 - Prob. 15TYKCh. 20 - Which of the following has the greatest potential...Ch. 20 - Prob. 17TYKCh. 20 - Petroleum-lysing bacteria are being engineered for...Ch. 20 - Prob. 19TYKCh. 20 - Prob. 20TYK
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- Which of the following characteristics is not true of a plasmid?a. It is a circular piece of DNA.b. It is required for normal cell function.c. It is found in bacteria.d. It can be transferred from cell to cell.arrow_forwardWhat do you mean by vector plasmid?arrow_forwardExplain why the genes whose products are required for the normal growth of bacteria not carried on plasmids? Give two examples (one bacterial gene and one plasmid gene) to support your answer.arrow_forward
- From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forwardThe polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNAarrow_forwardYou want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forward
- Choose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidarrow_forwardA recombinant plasmid can be used to a.) prevent nondisjunction at meiosis. b.) double the number of chromosomes in a plant cell. c.) cut DNA into restriction fragments. d.) transform a bacteriumarrow_forwardBacteria are frequently used in the generation of recombinant DNA molecules to: Group of answer choices A) Make copies of the vector + insert plasmid B) Ligate the insert into the vector C) Digest the vector for insert ligation D) Amplify the insert by PCRarrow_forward
- which of the following are considered as the main tools for dna technology? a. vectors b. plasmids c. organisms d. enzymesarrow_forwardExplain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmidarrow_forwardPlasmid vectors have the following characteristics, except _________ . a. MCS b. expression c. transfection d. single stranded e. cloningarrow_forward
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