Concept explainers
To review:
The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”
Introduction:
PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.
Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.
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Chapter 20 Solutions
Biological Science (7th Edition)
- Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? GGTAACC CCAATGG GGTTACC CCATTGGarrow_forwardFinding disease-causing mutations among the many DNAvariations that distinguish individual genomes involves____________ filtering of informationarrow_forwardThe FISH technique allows researchers to localize specificDNA sequences with respect to chromosomal bands or tovisualize entire chromosomes to facilitate_____________interpretationarrow_forward
- DNA polymerases used in PCR _______.arrow_forwardHershey and Chase were able to see whether the backteriophage moved genetic material because they used _____________ labels to attach to sulfur and phosphorus.arrow_forwardPlease write down the DNA sequence inferred from the below DNA gel. Shown are the products of a dideoxy sequencing reaction.arrow_forward
- The photograph provided is of a 1 μg of 2-log ladder indicating the molecular size in kilobases (Kb) and amount of DNA per band in ng. Estimate the concentration of your PCR product (amplicon) in ng/μL by visual comparison. The expected size of your amplicon is about 600-bp (0.6 kb). ____μg/μL?arrow_forwardDNA is visualized during agarose gel electrophoresis by ______________ . the fact that DNA fluoresces when illuminated with UV light the binding of a fluorescent dye that is easily detectable using radioactive antibodies that specifically bind to DNA the fact that DNA is blue and can be seen when millions of copies are present in a bandarrow_forwardPlease answer both Part a. The plasmid you used as a template in your positive control for your PCR is 3300 bp in size. Why is the PCR product only 650 bp long? b. You using PCR to check for the presence of the GFP gene, however the thermocyder (machine that through temperatures) has been misprogrammed and tge annealing temperature is set to 43c (low for your primers) Explain what you would expect to see on your gel and why?arrow_forward
- The most common procedure for cloning an animal is __________.arrow_forwardUse the sequence provided and make use of figure 1 to determine what restriction enzyme uses the spesific recognition site and figure 2 and 3 to detremine how many times does the sequence occur in the λ DNA sequence? GGATCC: __________________________________________________________ GAATTC: ___________________________________________________________ AAGCTT: ___________________________________________________________arrow_forwardA ____________ is required to transfer DNA sequences from one organism to another. reporter gene vector genetic probe restriction endonuclease genomic libraryarrow_forward
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