Biological Science (7th Edition)
7th Edition
ISBN: 9780134678320
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Greg Podgorski, Emily Taylor, Jeff Carmichael
Publisher: PEARSON
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Chapter 20, Problem 14PIAT
PROCESS OF SCIENCE Why was it important to include a positive control and a negative control in the PCR analysis?
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You are working in a molecular biology laboratory and are having challenges with your PCR. You decide to double-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effects would this mistake have on the PCR reaction? Suggest potential solution(s).
What does PCR stand for and just list the steps that are involved in it?
Draw your own PCR diagram to show the role of each component and relevance of each temperature shift during the PCR reaction.
What’s happening at each of those temperatures and why is it important it changes?
Chapter 20 Solutions
Biological Science (7th Edition)
Ch. 20 - Prob. 1TYKCh. 20 - What is a plasmid? a. an organelle found in many...Ch. 20 - CAUTION The human genome size is 3 billion base...Ch. 20 - Prob. 11PIATCh. 20 - Transgenic plants usually contain genes of...Ch. 20 - Prob. 13PIATCh. 20 - 14. PROCESS OF SCIENCE Why was it important to...Ch. 20 - Prob. 15PIATCh. 20 - Prob. 16PIAT
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- Describe the method of a PCR technique in which you can amplify fragments randomly? Briefly.arrow_forwardHow does PCR relate to biomedical engineering?arrow_forwardExplain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.arrow_forward
- What are the different types of PCR and their application? Please answer at your own words.arrow_forwardWhat is the difference between PCR and real-time PCR, and how do you explain it?arrow_forwardWhat would be the expected effect on the PCR reaction, if you increased the temperature of the annealing phase and the length of the elongation phase? O Accuracy will be reduced, but yield will be increased Accuracy and yield will be increased OAccuracy will be increased, but yield will be decreased Accuracy and yield will be reducedarrow_forward
- In PCR amplification why is it important to have a negative control?arrow_forwardPCR has revolutionalized biotechnology. Discuss three advantages and three disadvantages of PCR usage.arrow_forwardExplain the role of different polymerases in PCR with their advantages and limitations. Please explain at your own words.arrow_forward
- If Susie collects her cheek cells, but accidentally dumps the cell pellet into the waste container in step 4 of the DNA collection procedure, and doesn't realize it, what is the problem with her sample, moving forward, if any? No problem. Susie can expect the PCR reaction to work fine. Susie has no cells in her sample, so no DNA can be prepared from her sample. The PCR reaction will not contain template DNA.arrow_forwardWhat could you conclude from PCR/DGGE analysis of a samplethat yielded one band by PCR and one band by DGGE? Oneband by PCR and four bands by DGGE?arrow_forwardList the ingredients and describe how a PCR reaction worksarrow_forward
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