Gen Combo Ll Biology; Connect W/learnsmart Labs Access Card
4th Edition
ISBN: 9781259853197
Author: Robert Brooker
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 20, Problem 2TY
Summary Introduction
Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of the coding as well as the non-coding parts of DNA. The study of the genome is termed as genomics. Restriction enzymes are widely used enzymes in the field of genomics. These enzymes were discovered by Werner Arber, Hamilton Smith, and Daniel Nathans.
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Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
Arrange the following steps in the sequence they would happen in a DNA cloning experiment.
a. sealing DNA fragments into vectors with DNA ligase;
b. utilizing a probe to detect a clone in the library;
c. sequencing the clone's DNA;
d. creating a DNA library of clones;
e. cutting genomic DNA with restriction enzymes.
A.
e,a,d,b,c
B.
a,d,b,c,e
C.
c,b,e,a,d
D.
e,d,a,c,b
What is a restriction endonuclease?
Select one:
a. It is an enzyme that cleaves at a specific nucleotide sequence.
b. It restricts the movement of the DNA outside the nucleus.
c. It proofreads the DNA for accidental damages and corrects any errors.
d. It is an enzyme that separates the DNA double helix.
Chapter 20 Solutions
Gen Combo Ll Biology; Connect W/learnsmart Labs Access Card
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 1BCCh. 20.1 - Prob. 2CCCh. 20.1 - Prob. 3CCCh. 20.1 - Prob. 4CCCh. 20.1 - Prob. 2BCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.2 - Prob. 3CCCh. 20.3 - Prob. 1CC
Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.3 - Prob. 4CCCh. 20.3 - Prob. 1EQCh. 20.3 - Prob. 2EQCh. 20.3 - Prob. 3EQCh. 20 - Prob. 1TYCh. 20 - Prob. 2TYCh. 20 - DNA ligase is needed in a cloning experiment a. to...Ch. 20 - Prob. 4TYCh. 20 - Why is Taq polymerase used in PCR rather than...Ch. 20 - Lets suppose you want to clone a gene that has...Ch. 20 - Prob. 7TYCh. 20 - Prob. 8TYCh. 20 - Prob. 9TYCh. 20 - Prob. 10TYCh. 20 - Prob. 1CQCh. 20 - Prob. 2CQCh. 20 - Prob. 3CQCh. 20 - Identify and discuss three important advances that...Ch. 20 - Prob. 2COQ
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- The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA. Select one: True False Why? Select one: a. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are different. b. It must use same restriction endonuclease so that the restriction sites are identical in both foreign and bacterial DNA. c. It must use same restriction endonuclease so that the restriction sites are different in both foreign and bacterial DNA. d. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are exactly the same.arrow_forwardA has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forwardWhich of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forward
- Which of these conditions are always true of populations evolving due to natural selection? Condition 1: The population must vary in traits that are heritable. Condition 2: Some heritable traits must increase reproductive success. Condition 3: Individuals pass on most traits that they acquire during their lifetime. Select one: O a. Conditions 2 only O b. Conditions 1 and 2 Conditions 2 and 3 Conditions 1 only O c. O d.arrow_forwardRELPs: A. are the same length for mutant and normal beta-globin alleles. b. determine the sequence of bases in a DNA fragment. c. have short, palindromic sequences of DNA in the middle. D. are used as vectors. e. are produced by reaction with restriction enzymes and are detected with PCR and gel electrophoresis.arrow_forwardChromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular vectors (such as plasmids) using __________. a. Ligase; Restriction Endonucleases. b. Restriction endonucleases; Ligase. c. DNA polymerase 1; Primase. d. DNA polymerase 1; Ligase. e. Primase; Helicase.arrow_forward
- DNA cloning refers to a. making cloned bacteria. b. making cloned sheep or other eukaryotic organisms. c. how they made all those clones of strom troopers in Star Wars universe. d. probably all of the above, but generally, methods of making exact copies of DNA. e. none of the above.arrow_forwardA contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.arrow_forwardWhich of the following is a primary participant in cloning an isolated gene? a. restriction endonuclease b. vector c. host organism d. all of thesearrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardRestriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.arrow_forward
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