Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Chapter 20, Problem 2TYU

Which of the following tools of DNA technology is incorrectly paired with its use?

(A) electrophoresis—Separation of DNA fragments

(B) DNA ligase—cutting DNA, creating sticky ends of rcstric- tion fragments

(C) DNA polymerase—polvrnerase chain reaction to amplify Sections of DNA

(D) reverse transcriptase—production of cDNA from mRNA

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To amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the tube is 1) a buffer solution, 2) the DNA you want to amplify, 3) some DNA nucleotides, 4) a polymerase (like Taq polymerase), and O an RNA polymerase a set of forward and reverse primers some phospholipids for a cell membrane some ribosomes
In DNA technology, the term vector can refer to(A) the enzyme that cuts DNA into restrictionfragments.(B) the sticky end of a DNA fragment.(C) a SNP marker.(D) a plasmid used to transfer DNA into a living cell.
GENETICS if/when a "whole-genome shotgun" approach is used for DNA sequencing, which of the following is MOST likely to create problems during the assembly of a complete genomic sequence? a) long sequence reads b) a high degree of coverage/ redundancy in the sequence data c) the presence of repetitive DNA d) not enough contigs e) all of the above

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