Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Textbook Question
Chapter 20.1, Problem 4CC
VISUAL SKILLS Ø Compare Figure 20.7 with Figure 16.20. How does replication of DNA ends during PCR proceed without shortening the fragments each time?
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Transcribe and translate the DNA strand Remember to use the start and stop sequences.
ACGGTACCGTTAGCCGACATCGGGGACACTGACTCG
Learning Task 2: Make a model of a DNA template to determine the sequence of bases in th new DNA strands.
Then, answer the guide questions that follow.
Materials: crayons. Scissors, paste/tape, used folder or illustration board
Procedure:
Use the pattern of the DNA template: (attached to this LP). Color code, phosphate - blue, deoxyribose
sugar = green, nitrogenous base as follows: adenine= yellow, thymine pink, guanine = violet, cytosine
= red. And cut the shapes of each nucleotides.
Build a model of a strand of a DNA molecule. The strand should contain 6 base" rungs" following the given
order of the nucleotides: Guanine, Adenine, Cytosine, Thymine, Cytosine, Guanine.
%3D
%3D
Tape the cutout pattern to form the nucieotides. This will represent the left half of DNA.
Make a complementary strand that you made in step 3. Tape the cut -out pattern again forming the
nucieotides for the second strand of the DNA molecules.
Match the bases of the first strand and the second strand. Do not tape…
TOPIC: PCR and Gene Cloning Basics
Question: What are 2 possible roles of CaCl2 in the transformation process?
Chapter 20 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Ch. 20.1 - Prob. 1CCCh. 20.1 - DRAW IT One Strand of a DNA molecule has the...Ch. 20.1 - What are some potential difficulties in using...Ch. 20.1 - VISUAL SKILLS Compare Figure 20.7 with Figure...Ch. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - DNA technology has many medical applications....Ch. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 9TYUCh. 20 - MAKE CONNECTIONS Looking at Figure 20.15, what...Ch. 20 - DRAW IT You are cloning an aardvark gene, using a...Ch. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 13TYUCh. 20 - Prob. 14TYUCh. 20 - The water in the Yellowstone National Park hot...
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- please help with practice problemsarrow_forwardⒸ Macmillan Learning The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme Target sequence 5' GAATTC 3' 3' CTTAAG 5' EcoRI ECORV HaeIII HindIII PpUMI 5' GATATC 3' 3' CTATAG 5' EcoRI HindIII EcoRV HaeIII 5' GGCC 3' 3' CCGG 5' Incorrect 5' AAGCTT 3' 3' TTCGAA 5' 5' RGGWCCY 3' 3' YCCWGGR 5' 5' TCAGAATTCGGTGA 3' Cleavage 5' G 3' CTTAA 5' GAT 3' CTA 5' GG 3' CC AATTC 3' G5' ATC 3' TAG 5' CC 3' GG 5' AGCTT 3' A 5' Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' A 3' TTCGA 5' RG GWCCY 3' 3' YCCWG GR 5' 5' TCCAAGCTTGAATTC 3' EcoRV HaeIII HindIII EcoRI Incorrect Macmillan Learningarrow_forwardWhy should a student use the SQ3R method?arrow_forward
- Order the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of DNA to be sequenced add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase a primer binds to the single-stranded DNA template DNA polymerase extends the primer, incorporating deoxynucleotides a labeled dideoxynucleotide terminates the growing DNA chain gel electrophoresis separates the mixture of DNA fragments by size The DNA sequence is determined denature the double-stranded DNA Answer Bankarrow_forwardCorrect order ib which the following enzynes would operate to fix a damaged nucleotide in a human gene. a) nuclease, DNA polymerase, RNA primase b) helicase, DNA polymerase, DNA ligase c) DNA ligase, nuclease, helicase d) nuclease, DNA polymerase, DNA ligasearrow_forwardExpand PCR? Describe the different Steps involved in this technique?arrow_forward
- What's the main difference between first-generation sequencing, second-generation sequencing and whole genome amplification.arrow_forwardDNA Restriction, Electrophoresis 1. What are restriction enzymes? 2. How do restriction enzymes function? 3. Why are restriction enzymes important in biochemistry labs? 4. What is DNA Gel Electrophoresis? 5. How does DNA Gel Electrophoresis function? 6. Why is DNA Gel Electrophoresis important in biochemistry labs?arrow_forwardExperiment: dna restriction digestionarrow_forward
- Bonus: Which PCR process below generates an almost "instant signal" of a template in a sample using fluorescent reporter molecules? (this would speed up determining if nasal swab was positive or negative for coronavirus genetic material) O Western PCR O Eastern PCR O Real-Time or quantitative PCR O normal PCR O Southern PCRarrow_forwardGenetic Engineering Process (GEP) # 1: (What kind of process?) Picture A (Sequence #_ DNA introduced into bacterial cells Picture B (Sequence #, DNA ligase added, seals overhangs TTAA AATT AAT AATT TAA TTA TAA PATT PATT AATT recombinant DNA molecules Picture C (Sequence #. donor DNA vector vector and donor DNA digested (cleaved) with restriction enzyme AATT AATT 1477 AATT TTAA overhangs TTAA 1477 Picture D (Sequence #. AATT mixing recombinant DNA molecules replicate and cells dividearrow_forwardasap pleasearrow_forward
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