Exploring Chemical Analysis
Exploring Chemical Analysis
5th Edition
ISBN: 9781429275033
Author: Daniel C. Harris
Publisher: Macmillan Higher Education
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Chapter 23, Problem 23.21P
Interpretation Introduction

Interpretation:

log(Molecularweight) versus 1relative migrationtime has to be plotted. Molecular mass of ferritin light and heavy chains has to be plotted.

Concept Introduction:

The logarithm of molecular mass of the SDS-coated protein is proportional to 1relativeretentiontime of the protein through the gel. Absolute migration times are somewhat variable from run to run, so relative migration times are measured. The relative migration time is the migration time of a protein divided by the migration time of a fast-moving small dye molecule.

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