Concept explainers
Case Summary:
Consensus sequences are sequences that occur very frequently. These are present on region upstream of the gene (Promoter). To prove this, in-vitro experiment can be performed by engineering mutation in the consensus sequences present 25- 30bp above the transcription start site.
Characters of the case:
Promoters are regions to which RNA polymerases bind to initiate transcription. Eukaryote has promoter TATA box 25-30 base pair upstream of the transcription start site and prokaryotes have Shine-Dalgarnosequence.
Interpretation:
As discussed in the text, promoters were originally identified as consensus sequences upstream from the transcriptional start sites. The additional evidence that might support the assignment of these sequences as parts of promoters has to be found.
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