WORLD OF CELL+MASTERING ACCESS >CUSTOM
WORLD OF CELL+MASTERING ACCESS >CUSTOM
9th Edition
ISBN: 9781323445044
Author: Hardin
Publisher: PEARSON C
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Chapter 25, Problem 25.11PS

(a)

Summary Introduction

To determine: The reason that a recA protein mutation in Escherichia coli make it a better host for the propagation of recombinant plasmid DNA (Deoxyribose nucleic acid).

Introduction: Restriction endonucleases which are also called molecular scissors are the enzymes that are used in molecular biology for the formation of the recombinant molecule. Plasmid vectors act as the carriers of foreign information that has to be incorporated into a recombinant molecule.

(b)

Summary Introduction

To determine: The reason that mutations in restriction endonucleases of Escherichia coli are useful.

Introduction: Restriction endonucleases which are also called molecular scissors are the enzymes that are used in molecular biology for the formation of the recombinant molecule. Plasmid vectors act as the carriers of foreign information that has to be incorporated into a recombinant molecule.

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Cloning vectors for E Coli.    For each of the following items, describe what it is explain the necessity for each in cloning plasmids that are used for the expression (production) of protein.  what would happen if this element was omitted?  The Multiple Cloning Site (MCS) Origin of replication –  (AmpR) antibiotic resistance gene – T7 promoter -  T7 termination site-  lac I gene
Transformation and sequence validation of the clones
Question:- Explain why there are very few sequence-specific DNA-binding proteins that bind to the minor groove of double-stranded DNA.
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